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AFM Force Spectroscopy Reveals the Role of Integrins and Their Activation in Cell-Biomaterial Interactions

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Harjumäki, Riina
dc.contributor.author Zhang, Xue
dc.contributor.author Nugroho, Robertus Wahyu N.
dc.contributor.author Farooq, Muhammad
dc.contributor.author Lou, Yan Ru
dc.contributor.author Yliperttula, Marjo
dc.contributor.author Valle-Delgado, Juan José
dc.contributor.author Österberg, Monika
dc.date.accessioned 2020-11-30T08:14:14Z
dc.date.available 2020-11-30T08:14:14Z
dc.date.issued 2020-03-16
dc.identifier.citation Harjumäki , R , Zhang , X , Nugroho , R W N , Farooq , M , Lou , Y R , Yliperttula , M , Valle-Delgado , J J & Österberg , M 2020 , ' AFM Force Spectroscopy Reveals the Role of Integrins and Their Activation in Cell-Biomaterial Interactions ' , ACS Applied Bio Materials , vol. 3 , no. 3 , pp. 1406-1417 . https://doi.org/10.1021/acsabm.9b01073 en
dc.identifier.issn 2576-6422
dc.identifier.other PURE UUID: 554dc5c5-9a31-456b-9f9d-d1722f466fa5
dc.identifier.other PURE ITEMURL: https://research.aalto.fi/en/publications/afm-force-spectroscopy-reveals-the-role-of-integrins-and-their-activation-in-cellbiomaterial-interactions(554dc5c5-9a31-456b-9f9d-d1722f466fa5).html
dc.identifier.other PURE LINK: http://www.scopus.com/inward/record.url?scp=85080122441&partnerID=8YFLogxK
dc.identifier.other PURE FILEURL: https://research.aalto.fi/files/53468219/acsabm.9b01073.pdf
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/61702
dc.description.abstract Transmembrane protein integrins play a key role in cell adhesion. Cell-biomaterial interactions are affected by integrin expression and conformation, which are actively controlled by cells. Although integrin structure and function have been studied in detail, quantitative analyses of integrin-mediated cell-biomaterial interactions are still scarce. Here, we have used atomic force spectroscopy to study how integrin distribution and activation (via intracellular mechanisms in living cells or by divalent cations) affect the interaction of human pluripotent stem cells (WA07) and human hepatocarcinoma cells (HepG2) with promising biomaterials-human recombinant laminin-521 (LN-521) and cellulose nanofibrils (CNF). Cell adhesion to LN-521-coated probes was remarkably influenced by cell viability, divalent cations, and integrin density in WA07 colonies, indicating that specific bonds between LN-521 and activated integrins play a significant role in the interactions between LN-521 and HepG2 and WA07 cells. In contrast, the interactions between CNF and cells were nonspecific and not influenced by cell viability or the presence of divalent cations. These results shed light on the underlying mechanisms of cell adhesion, with direct impact on cell culture and tissue engineering applications. en
dc.format.extent 12
dc.format.extent 1406-1417
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries ACS Applied Bio Materials en
dc.relation.ispartofseries Volume 3, issue 3 en
dc.rights openAccess en
dc.title AFM Force Spectroscopy Reveals the Role of Integrins and Their Activation in Cell-Biomaterial Interactions en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Department of Bioproducts and Biosystems
dc.contributor.department Bioproduct Chemistry
dc.contributor.department Bio-based Colloids and Materials
dc.contributor.department University of Helsinki
dc.subject.keyword atomic force microscope
dc.subject.keyword cellulose nanofibrils
dc.subject.keyword colloidal probe microscopy
dc.subject.keyword force spectroscopy
dc.subject.keyword human hepatocarcinoma cells
dc.subject.keyword human pluripotent stem cells
dc.subject.keyword integrin
dc.subject.keyword laminin-521
dc.identifier.urn URN:NBN:fi:aalto-2020113020547
dc.identifier.doi 10.1021/acsabm.9b01073
dc.type.version publishedVersion

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