An electroanalytical toolbox for bioapplications

 |  Login

Show simple item record

dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Carlsson, Sanna
dc.date.accessioned 2012-08-23T11:32:11Z
dc.date.available 2012-08-23T11:32:11Z
dc.date.issued 2009
dc.identifier.isbn 978-952-248-090-3
dc.identifier.isbn 978-952-248-089-7 (printed) #8195;
dc.identifier.issn 1795-4584
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/4693
dc.description.abstract This thesis describes the utilization of electrochemical methods for studying polyion-surfactant interactions, drug transfer through biomimetic phospholipid membranes and lateral charge transfer in DNA. Different aspects of oligonucleotide drug development and the corresponding delivery problem are considered and thus, the literature review in the beginning of the thesis is divided into four parts, giving an overview of the research field. The problem of oligonucleotide delivery has been approached using a surface-active compound as a carrier for the oligonucleotide. The complexation of the oligonucleotide with the surfactant shields most of the oligonucleotide's negative charges, thus making its incorporation into the lipophilic cell membrane and transfer through it more probable. Thermodynamics and kinetics of surfactant-oligonucleotide binding have been measured using advanced electrochemical methods based on the electrified liquid-liquid interface. A micropipette-based technique provided information on the stability and energetics of surfactant-oligonucleotide complexes, as well as the degree of binding. The kinetics of the surfactant-oligonucleotide interaction has been approached with a hydrodynamic technique enabling the control of the residence time of surfactant and oligonucleotide. Membrane activity is the most important property of the oligonucleotide-carrier complex. Carrier complexes should penetrate the biological phospholipid membrane, then escape the interior of the lipid membrane and transfer to the interior of the cell. Membrane activity of the surfactant-oligonucleotide complexes has been studied using a biomimetic phospholipid monolayer immobilised at an electrified liquid-liquid interface. In a related study, charge transfer in DNA immobilized on silicon surface has been studied by scanning electrochemical microscopy (SECM) to get fundamental information on nano-sized immobilized DNA-architectures. en
dc.format.extent Verkkokirja (1664 KB, 71 s.)
dc.format.mimetype application/pdf
dc.language.iso en en
dc.publisher Teknillinen korkeakoulu en
dc.relation.ispartofseries TKK dissertations, 182 en
dc.relation.haspart [Publication 1]: Sanna Hakkarainen, Sarah L. Gilbert, Anna-Kaisa Kontturi, and Kyösti Kontturi. 2004. Amperometric method for determining the degree of complexation of polyelectrolytes with cationic surfactants. Journal of Colloid and Interface Science, volume 272, number 2, pages 404-410. © 2004 Elsevier Science. By permission. en
dc.relation.haspart [Publication 2]: Sanna Hakkarainen, Anna-Kaisa Kontturi, Kyösti Kontturi, and Arto Urtti. 2004. Electrochemical and calorimetric study of oligonucleotide complexation with cetylpyridinium chloride. European Journal of Pharmaceutical Sciences, volume 23, numbers 4-5, pages 371-377. © 2004 Elsevier Science. By permission. en
dc.relation.haspart [Publication 3]: Sanna Carlsson, Peter Liljeroth, and Kyösti Kontturi. 2005. Channel flow configuration for studying the kinetics of surfactant–polyelectrolyte binding. Analytical Chemistry, volume 77, number 21, pages 6895-6901. en
dc.relation.haspart [Publication 4]: Sanna Carlsson, Anna-Kaisa Kontturi, and Kyösti Kontturi. 2006. Improving membrane activity of oligonucleotides by cetylpyridinium chloride: An electrochemical study. European Journal of Pharmaceutical Sciences, volume 29, number 5, pages 451-459. © 2006 Elsevier Science. By permission. en
dc.relation.haspart [Publication 5]: Lars H. Lie, Michael V. Mirkin, Sanna Hakkarainen, Andrew Houlton, and Benjamin R. Horrocks. 2007. Electrochemical detection of lateral charge transport in metal complex-DNA monolayers synthesized on Si(1 1 1) electrodes. Journal of Electroanalytical Chemistry, volume 603, number 1, pages 67-80. © 2007 Elsevier Science. By permission. en
dc.subject.other Biotechnology
dc.title An electroanalytical toolbox for bioapplications en
dc.type G5 Artikkeliväitöskirja fi
dc.contributor.department Kemian laitos fi
dc.subject.keyword oligonucleotide en
dc.subject.keyword DNA en
dc.subject.keyword polyelectrolyte en
dc.identifier.urn URN:ISBN:978-952-248-090-3
dc.type.dcmitype text en
dc.type.ontasot Väitöskirja (artikkeli) fi
dc.type.ontasot Doctoral dissertation (article-based) en


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search archive


Advanced Search

article-iconSubmit a publication

Browse

My Account