Dual-light photodynamic therapy administered daily provides a sustained antibacterial effect on biofilm and prevents Streptococcus mutans adaptation

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Nikinmaa, Sakari
dc.contributor.author Alapulli, Heikki
dc.contributor.author Auvinen, Petri
dc.contributor.author Vaara, Martti
dc.contributor.author Rantala, Juha
dc.contributor.author Kankuri, Esko
dc.contributor.author Sorsa, Timo
dc.contributor.author Meurman, Jukka
dc.contributor.author Pätilä, Tommi
dc.date.accessioned 2020-06-25T08:37:11Z
dc.date.available 2020-06-25T08:37:11Z
dc.date.issued 2020-05
dc.identifier.citation Nikinmaa , S , Alapulli , H , Auvinen , P , Vaara , M , Rantala , J , Kankuri , E , Sorsa , T , Meurman , J & Pätilä , T 2020 , ' Dual-light photodynamic therapy administered daily provides a sustained antibacterial effect on biofilm and prevents Streptococcus mutans adaptation ' , PloS one , vol. 15 , no. 5 , e0232775 . https://doi.org/10.1371/journal.pone.0232775 en
dc.identifier.issn 1932-6203
dc.identifier.other PURE UUID: 3629442d-e56a-42ca-8663-e0d204af6791
dc.identifier.other PURE ITEMURL: https://research.aalto.fi/en/publications/duallight-photodynamic-therapy-administered-daily-provides-a-sustained-antibacterial-effect-on-biofilm-and-prevents-streptococcus-mutans-adaptation(3629442d-e56a-42ca-8663-e0d204af6791).html
dc.identifier.other PURE LINK: http://www.scopus.com/inward/record.url?scp=85084315537&partnerID=8YFLogxK
dc.identifier.other PURE FILEURL: https://research.aalto.fi/files/43347969/Nikinmaa_Dual_light.journal.pone.0232775_1.pdf
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/45108
dc.description.abstract Antibacterial photodynamic therapy (aPDT) and antibacterial blue light (aBL) are emerging treatment methods auxiliary to mechanical debridement for periodontitis. APDT provided with near-infrared (NIR) light in conjunction with an indocyanine green (ICG) photosensitizer has shown efficacy in several dental in-office-treatment protocols. In this study, we tested Streptococcus mutans biofilm sensitivity to either aPDT, aBL or their combination dual-light aPDT (simultaneous aPDT and aBL) exposure. Biofilm was cultured by pipetting diluted Streptococcus mutans suspension with growth medium on the bottom of well plates. Either aPDT (810 nm) or aBL (405 nm) or a dual-light aPDT (simultaneous 810 nm aPDT and 405 nm aBL) was applied with an ICG photosensitizer in cases of aPDT or dual-light, while keeping the total given radiant exposure constant at 100 J/cm2. Single-dose light exposures were given after one-day or four-day biofilm incubations. Also, a model of daily treatment was provided by repeating the same lightdose daily on four-day and fourteen-day biofilm incubations. Finally, the antibacterial action of the dual-light aPDT with different energy ratios of 810 nm and 405 nm of light were examined on the single-day and four-day biofilm protocols. At the end of each experiment the bacterial viability was assessed by colony-forming unit method. Separate samples were prepared for confocal 3D biofilm imaging. On a one-day biofilm, the dual-light aPDT was significantly more efficient than aBL or aPDT, although all modalities were bactericidal. On a four-day biofilm, a single exposure of aPDT or dual-light aPDT was more efficient than aBL, resulting in a four logarithmic scale reduction in bacterial counts. Surprisingly, when the same amount of aPDT was repeated daily on a four-day or a fourteen-day biofilm, bacterial viability improved significantly. A similar improvement in bacterial viability was observed after repetitive aBL application. This viability improvement was eliminated when dual-light aPDT was applied. By changing the 405 nm to 810 nm radiant exposure ratio in dual-light aPDT, the increase in aBL improved the antibacterial action when the biofilm was older. In conclusion, when aPDT is administered repeatedly to S. mutans biofilm, a single wavelength-based aBL or aPDT leads to a significant biofilm adaptation and increased S. mutans viability. The combined use of aBL light in synchrony with aPDT arrests the adaptation and provides significantly improved and sustained antibacterial efficacy. en
dc.format.mimetype application/pdf
dc.language.iso en en
dc.publisher Public Library of Science
dc.relation.ispartofseries PloS one en
dc.relation.ispartofseries Volume 15, issue 5 en
dc.rights openAccess en
dc.subject.other Biochemistry, Genetics and Molecular Biology(all) en
dc.subject.other Agricultural and Biological Sciences(all) en
dc.subject.other General en
dc.subject.other 1182 Biochemistry, cell and molecular biology en
dc.title Dual-light photodynamic therapy administered daily provides a sustained antibacterial effect on biofilm and prevents Streptococcus mutans adaptation en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Department of Neuroscience and Biomedical Engineering
dc.contributor.department University of Helsinki
dc.contributor.department Northern Antibiotics Ltd.
dc.contributor.department Koite Health Oy
dc.contributor.department Karolinska Institutet
dc.contributor.department Department of Neuroscience and Biomedical Engineering en
dc.subject.keyword Biochemistry, Genetics and Molecular Biology(all)
dc.subject.keyword Agricultural and Biological Sciences(all)
dc.subject.keyword General
dc.subject.keyword 1182 Biochemistry, cell and molecular biology
dc.identifier.urn URN:NBN:fi:aalto-202006254065
dc.identifier.doi 10.1371/journal.pone.0232775
dc.type.version publishedVersion

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