Multivalent dendrons for high-affinity DNA binding

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Kostiainen, Mauri
dc.date.accessioned 2012-08-20T06:29:44Z
dc.date.available 2012-08-20T06:29:44Z
dc.date.issued 2008
dc.identifier.isbn 978-951-22-9372-8
dc.identifier.isbn 978-951-22-9371-1 (printed) #8195;
dc.identifier.issn 1795-4584
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/4492
dc.description.abstract In this Thesis the synthesis of various Newkome-type polyamine dendrons and their DNA binding properties is presented. These cationic dendrons bind DNA with extremely high affinity through multivalent ionic interactions. Dendrons with o-nitrobenzyl linked surface groups can be cleaved from the dendron framework by optical irradiation resulting in rapid release of the covalently bound surface groups and non-covalently bound DNA, due to dendron degradation and charge switching multivalency. N-maleimido cored dendrons can be attached onto protein surfaces in site-specific manner to yield exactly defined one-to-one protein-polymer conjugates, where the number of dendrons and their attachment site on the protein surface is precisely known. The resulting protein-dendron conjugates bind DNA with high affinity. Further studies in gene transfection, cytotoxicity and self-assembly establish relevance in gene therapy and surface patterning. en
dc.format.extent Verkkokirja (2975 KB, 52,[5] s.)
dc.format.mimetype application/pdf
dc.language.iso en en
dc.publisher Teknillinen korkeakoulu en
dc.relation.ispartofseries TKK dissertations, 122 en
dc.relation.haspart [Publication 1]: Mauri A. Kostiainen, John G. Hardy, and David K. Smith. 2005. High-affinity multivalent DNA binding by using low-molecular-weight dendrons. Angewandte Chemie International Edition, volume 44, number 17, pages 2556-2559. en
dc.relation.haspart [Publication 2]: John G. Hardy, Mauri A. Kostiainen, David K. Smith, Nathan P. Gabrielson, and Daniel W. Pack. 2006. Dendrons with spermine surface groups as potential building blocks for nonviral vectors in gene therapy. Bioconjugate Chemistry, volume 17, number 1, pages 172-178. en
dc.relation.haspart [Publication 3]: Mauri A. Kostiainen, David K. Smith, and Olli Ikkala. 2007. Optically triggered release of DNA from multivalent dendrons by degrading and charge-switching multivalency. Angewandte Chemie International Edition, volume 46, number 40, pages 7600-7604. en
dc.relation.haspart [Publication 4]: Mauri A. Kostiainen, Géza R. Szilvay, David K. Smith, Markus B. Linder, and Olli Ikkala. 2006. Multivalent dendrons for high-affinity adhesion of proteins to DNA. Angewandte Chemie International Edition, volume 45, number 21, pages 3538-3542. en
dc.relation.haspart [Publication 5]: Mauri A. Kostiainen, Géza R. Szilvay, Julia Lehtinen, David K. Smith, Markus B. Linder, Arto Urtti, and Olli Ikkala. 2007. Precisely defined protein-polymer conjugates: Construction of synthetic DNA binding domains on proteins by using multivalent dendrons. ACS Nano, volume 1, number 2, pages 103-113. en
dc.subject.other Materials science en
dc.title Multivalent dendrons for high-affinity DNA binding en
dc.type G5 Artikkeliväitöskirja fi
dc.contributor.department Teknillisen fysiikan laitos fi
dc.subject.keyword self-assembly en
dc.subject.keyword dendrimers en
dc.subject.keyword dendron en
dc.subject.keyword DNA en
dc.subject.keyword protein en
dc.identifier.urn URN:ISBN:978-951-22-9372-8
dc.type.dcmitype text en
dc.type.ontasot Väitöskirja (artikkeli) fi
dc.type.ontasot Doctoral dissertation (article-based) en


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