Learning Centre

Different effects of carbohydrate binding modules on the viscoelasticity of nanocellulose gels

 |  Login

Show simple item record

dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Rooijakkers, Bart J.M.
dc.contributor.author Arola, Suvi
dc.contributor.author Velagapudi, Rama
dc.contributor.author Linder, Markus B.
dc.date.accessioned 2020-04-30T12:50:08Z
dc.date.available 2020-04-30T12:50:08Z
dc.date.issued 2020-07
dc.identifier.citation Rooijakkers , B J M , Arola , S , Velagapudi , R & Linder , M B 2020 , ' Different effects of carbohydrate binding modules on the viscoelasticity of nanocellulose gels ' , Biochemistry and Biophysics Reports , vol. 22 , 100766 . https://doi.org/10.1016/j.bbrep.2020.100766 en
dc.identifier.issn 2405-5808
dc.identifier.other PURE UUID: 63c9f0b7-2d7a-4ed2-9b13-6dda2e774f9b
dc.identifier.other PURE ITEMURL: https://research.aalto.fi/en/publications/63c9f0b7-2d7a-4ed2-9b13-6dda2e774f9b
dc.identifier.other PURE LINK: http://www.scopus.com/inward/record.url?scp=85083322399&partnerID=8YFLogxK
dc.identifier.other PURE FILEURL: https://research.aalto.fi/files/42576439/CHEM_Rooijakkers_et_al_2020_Different_effects_BioRep.pdf
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/43999
dc.description.abstract Many cellulose degrading and modifying enzymes have distinct parts called carbohydrate binding modules (CBMs). The CBMs have been shown to increase the concentration of enzymes on the insoluble substrate and thereby enhance catalytic activity. It has been suggested that CBMs also have a role in disrupting or dispersing the insoluble cellulose substrate, but dispute remains and explicit evidence of such a mechanism is lacking. We produced the isolated CBMs from two major cellulases (Cel6A and Cel7A) from Trichoderma reesei as recombinant proteins in Escherichia coli. We then studied the viscoelastic properties of native unmodified cellulose nanofibrils (CNF) in combination with the highly purified CBMs to detect possible functional effects of the CBMs on the CNF. The two CBMs showed clearly different effects on the viscoelastic properties of CNF. The difference in effects is noteworthy, yet it was not possible to conclude for example disruptive effects. We discuss here the alternative explanations for viscoelastic effects on CNF caused by CBMs, including the effect of ionic cosolutes. en
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries Biochemistry and Biophysics Reports en
dc.relation.ispartofseries Volume 22 en
dc.rights openAccess en
dc.title Different effects of carbohydrate binding modules on the viscoelasticity of nanocellulose gels en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Department of Bioproducts and Biosystems
dc.contributor.department VTT Technical Research Centre of Finland
dc.subject.keyword CBM
dc.subject.keyword Cellulose nanofibril
dc.subject.keyword Family 1 carbohydrate binding module
dc.subject.keyword Gel
dc.subject.keyword Rheology
dc.identifier.urn URN:NBN:fi:aalto-202004302978
dc.identifier.doi 10.1016/j.bbrep.2020.100766
dc.type.version publishedVersion

Files in this item

Files Size Format View

There are no open access files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search archive

Advanced Search

article-iconSubmit a publication