Calcium Dependent Reversible Aggregation of Escherichia coli Biomimicking Vesicles Enables Formation of Supported Vesicle Layers on Silicon Dioxide

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en Dusa, Filip Chen, Wen Witos, Joanna Wiedmer, Susanne K. 2019-04-02T06:52:41Z 2019-04-02T06:52:41Z 2019-03-01
dc.identifier.citation Dusa , F , Chen , W , Witos , J & Wiedmer , S K 2019 , ' Calcium Dependent Reversible Aggregation of Escherichia coli Biomimicking Vesicles Enables Formation of Supported Vesicle Layers on Silicon Dioxide ' Frontiers in materials , vol. 6 , 23 . en
dc.identifier.issn 2296-8016
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dc.identifier.other PURE ITEMURL:
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dc.description.abstract The importance of using biomimicking membranes for various biological applications is rising, as such models are relevant for imitating real organisms. In addition, biomimicking membranes are usually much more repeatable in preparation and easier to handle during analysis than real organisms or biological membranes. In this work, we developed a method for the adsorption of intact small unilamellar Escherichia coli (E. cols) vesicles (Z-average size of 73 nm) on SiO2 substrate material. We describe the adsorption process based on the use of two surface sensitive techniques, i.e., nanoplasmonic sensing (NPS) and quartz crystal microbalance (QCM). The acquired data show that the adsorption follows a two-step process. The first step is a slow adsorption of E coil vesicle aggregates held together by 5 mM of calcium (Z-average size of 531 nm). The Z-average of the aggregates decreased almost three times when the calcium concentration was decreased to 0.1 mM. This suggests that the aggregates were disassembling to some extent when calcium was removed from the system. With both techniques, i.e., NPS and QCM, we observed a second rapid adsorption step after the solution was changed to deionized water. In this second step, the aggregates started to fall apart as the calcium concentration dropped, and the released vesicles started to adsorb onto unoccupied spots at the SiO2 surface of the sensors. Extensive release of mass from the surface was confirmed by QCM, where it was reflected by a sharp increase of frequency, while NPS, due to its lower sensing depth of a few tens of nanometers, did not record such a change. Taken together, we have developed a protocol to form a supported vesicle layer (SVL) of E coli vesicles on SiO2 surface using sodium 4-(2-hydroxyethyppiperazine-1-ethanesulfonate buffer, thus enabling the preparation of E coli biomimicking SVLs for interaction studies of compounds of interest. The immobilization happens via a two-step adsorption process. en
dc.format.extent 8
dc.format.mimetype application/pdf
dc.language.iso en en
dc.publisher Frontiers Media S.A
dc.relation.ispartofseries Frontiers in materials en
dc.relation.ispartofseries Volume 6 en
dc.rights openAccess en
dc.subject.other Materials Science (miscellaneous) en
dc.subject.other 215 Chemical engineering en
dc.title Calcium Dependent Reversible Aggregation of Escherichia coli Biomimicking Vesicles Enables Formation of Supported Vesicle Layers on Silicon Dioxide en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Czech Academy of Sciences
dc.contributor.department University of Helsinki
dc.contributor.department Biorefineries
dc.contributor.department Department of Bioproducts and Biosystems en
dc.subject.keyword Aggregation
dc.subject.keyword Biomembrane
dc.subject.keyword Escherichia coli
dc.subject.keyword Nanoplasmonic sensing
dc.subject.keyword Quartz crystal microbalance
dc.subject.keyword Vesicles
dc.subject.keyword CARDIOLIPIN
dc.subject.keyword MEMBRANE
dc.subject.keyword ANIONIC LIPOSOMES
dc.subject.keyword quartz crystal microbalance
dc.subject.keyword aggregation
dc.subject.keyword BACTERIAL
dc.subject.keyword BILAYERS
dc.subject.keyword MODEL
dc.subject.keyword ADSORPTION
dc.subject.keyword DEFORMATION
dc.subject.keyword CATIONS
dc.subject.keyword vesicles
dc.subject.keyword nanoplasmonic sensing
dc.subject.keyword biomembrane
dc.subject.keyword Materials Science (miscellaneous)
dc.subject.keyword 215 Chemical engineering
dc.identifier.urn URN:NBN:fi:aalto-201904022410
dc.identifier.doi 10.3389/fmats.2019.00023
dc.type.version publishedVersion

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