Biocatalytic Production of Amino Carbohydrates through Oxidoreductase and Transaminase Cascades

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en Aumala, Ville Mollerup, Filip Jurak, Edita Blume, Fabian Karppi, Johanna Koistinen, Antti E. Schuiten, Eva Voß, Moritz Bornscheuer, Uwe Deska, Jan Master, Emma R. 2019-02-25T08:50:24Z 2019-02-25T08:50:24Z 2019-01-01
dc.identifier.citation Aumala , V , Mollerup , F , Jurak , E , Blume , F , Karppi , J , Koistinen , A E , Schuiten , E , Voß , M , Bornscheuer , U , Deska , J & Master , E R 2019 , ' Biocatalytic Production of Amino Carbohydrates through Oxidoreductase and Transaminase Cascades ' ChemSusChem . en
dc.identifier.issn 1864-5631
dc.identifier.issn 1864-564X
dc.identifier.other PURE UUID: 9cffd97b-465b-4d0c-8b24-b2adf27846fa
dc.identifier.other PURE ITEMURL:
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dc.description | openaire: EC/H2020/648925/EU//BHIVE
dc.description.abstract Plant-derived carbohydrates are an abundant renewable resource. Transformation of carbohydrates into new products, including amine-functionalized building blocks for biomaterials applications, can lower reliance on fossil resources. Herein, biocatalytic production routes to amino carbohydrates, including oligosaccharides, are demonstrated. In each case, two-step biocatalysis was performed to functionalize d-galactose-containing carbohydrates by employing the galactose oxidase from Fusarium graminearum or a pyranose dehydrogenase from Agaricus bisporus followed by the ω-transaminase from Chromobacterium violaceum (Cvi-ω-TA). Formation of 6-amino-6-deoxy-d-galactose, 2-amino-2-deoxy-d-galactose, and 2-amino-2-deoxy-6-aldo-d-galactose was confirmed by mass spectrometry. The activity of Cvi-ω-TA was highest towards 6-aldo-d-galactose, for which the highest yield of 6-amino-6-deoxy-d-galactose (67 %) was achieved in reactions permitting simultaneous oxidation of d-galactose and transamination of the resulting 6-aldo-d-galactose. en
dc.format.mimetype application/pdf
dc.language.iso en en
dc.publisher Wiley-VCH Verlag
dc.relation info:eu-repo/grantAgreement/EC/H2020/648925/EU//BHIVE
dc.relation.ispartofseries ChemSusChem en
dc.rights openAccess en
dc.subject.other Environmental Chemistry en
dc.subject.other Organic Chemistry en
dc.subject.other Biochemistry en
dc.subject.other Biotechnology en
dc.subject.other 116 Chemical sciences en
dc.subject.other 1182 Biochemistry, cell and molecular biology en
dc.subject.other 1 Natural sciences en
dc.subject.other Organic chemistry en
dc.subject.other Biochemistry, biophysics en
dc.subject.other biomaterials en
dc.title Biocatalytic Production of Amino Carbohydrates through Oxidoreductase and Transaminase Cascades en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Department of Bioproducts and Biosystems
dc.contributor.department University of Groningen
dc.contributor.department Department of Chemistry and Materials Science
dc.contributor.department Protein Technology
dc.contributor.department University of Greifswald
dc.subject.keyword amination
dc.subject.keyword biocatalysis
dc.subject.keyword carbohydrates
dc.subject.keyword domino reactions
dc.subject.keyword enzymes
dc.subject.keyword Environmental Chemistry
dc.subject.keyword Organic Chemistry
dc.subject.keyword Biochemistry
dc.subject.keyword Biotechnology
dc.subject.keyword 116 Chemical sciences
dc.subject.keyword 1182 Biochemistry, cell and molecular biology
dc.subject.keyword 1 Natural sciences
dc.subject.keyword Organic chemistry
dc.subject.keyword Biochemistry, biophysics
dc.subject.keyword biomaterials
dc.identifier.urn URN:NBN:fi:aalto-201902251991
dc.identifier.doi 10.1002/cssc.201802580
dc.type.version publishedVersion

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