Effects of design choices on the stiffness of wireframe DNA origami structures

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Benson, Erik
dc.contributor.author Mohammed, Abdulmelik
dc.contributor.author Rayneau-Kirkhope, Daniel
dc.contributor.author Gådin, Andreas
dc.contributor.author Orponen, Pekka
dc.contributor.author Högberg, Björn
dc.date.accessioned 2019-01-30T15:07:20Z
dc.date.available 2019-01-30T15:07:20Z
dc.date.issued 2018-09-25
dc.identifier.citation Benson , E , Mohammed , A , Rayneau-Kirkhope , D , Gådin , A , Orponen , P & Högberg , B 2018 , ' Effects of design choices on the stiffness of wireframe DNA origami structures ' ACS Nano , vol. 12 , no. 9 , pp. 9291-9299 . https://doi.org/10.1021/acsnano.8b04148 en
dc.identifier.issn 1936-0851
dc.identifier.issn 1936-086X
dc.identifier.other PURE UUID: 1738ee10-316a-4d15-93d7-dfee8beea6f7
dc.identifier.other PURE ITEMURL: https://research.aalto.fi/en/publications/effects-of-design-choices-on-the-stiffness-of-wireframe-dna-origami-structures(1738ee10-316a-4d15-93d7-dfee8beea6f7).html
dc.identifier.other PURE LINK: http://www.scopus.com/inward/record.url?scp=85053519407&partnerID=8YFLogxK
dc.identifier.other PURE FILEURL: https://research.aalto.fi/files/31144493/dnarods_2018.pdf
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/36224
dc.description.abstract DNA origami is a powerful method for the creation of 3D nanoscale objects, and in the past few years, interest in wireframe origami designs has increased due to their potential for biomedical applications. In DNA wireframe designs, the construction material is double-stranded DNA, which has a persistence length of around 50 nm. In this work, we study the effect of various design choices on the stiffness versus final size of nanoscale wireframe rods, given the constraints on origami designs set by the DNA origami scaffold size. An initial theoretical analysis predicts two competing mechanisms limiting rod stiffness, whose balancing results in an optimal edge length. For small edge lengths, the bending of the rod's overall frame geometry is the dominant factor, while the flexibility of individual DNA edges has a greater contribution at larger edge lengths. We evaluate our design choices through simulations and experiments and find that the stiffness of the structures increases with the number of sides in the cross-section polygon and that there are indications of an optimal member edge length. We also ascertain the effect of nicked DNA edges on the stiffness of the wireframe rods and demonstrate that ligation of the staple breakpoint nicks reduces the observed flexibility. Our simulations also indicate that the persistence length of wireframe DNA structures significantly decreases with increasing monovalent salt concentration. en
dc.format.extent 9
dc.format.extent 9291-9299
dc.format.mimetype application/pdf
dc.language.iso en en
dc.publisher AMERICAN CHEMICAL SOCIETY
dc.relation.ispartofseries ACS Nano en
dc.relation.ispartofseries Volume 12, issue 9 en
dc.rights openAccess en
dc.subject.other Materials Science(all) en
dc.subject.other Engineering(all) en
dc.subject.other Physics and Astronomy(all) en
dc.subject.other Computer Science Applications en
dc.subject.other 114 Physical sciences en
dc.subject.other 113 Computer and information sciences en
dc.subject.other Theoretical computer science en
dc.title Effects of design choices on the stiffness of wireframe DNA origami structures en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Karolinska Institutet
dc.contributor.department Department of Computer Science
dc.contributor.department Molecular Materials
dc.contributor.department Department of Applied Physics en
dc.subject.keyword beam theory
dc.subject.keyword DNA nanostructures
dc.subject.keyword DNA origami
dc.subject.keyword oxDNA
dc.subject.keyword persistence length
dc.subject.keyword rigidity
dc.subject.keyword wireframe DNA origami
dc.subject.keyword Materials Science(all)
dc.subject.keyword Engineering(all)
dc.subject.keyword Physics and Astronomy(all)
dc.subject.keyword Computer Science Applications
dc.subject.keyword 114 Physical sciences
dc.subject.keyword 113 Computer and information sciences
dc.subject.keyword Theoretical computer science
dc.identifier.urn URN:NBN:fi:aalto-201901301394
dc.identifier.doi 10.1021/acsnano.8b04148
dc.type.version acceptedVersion


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