Direct analysis by time-of-flight secondary ion mass spectrometry reveals action of bacterial laccase-mediator systems on both hardwood and softwood samples

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Goacher, Robyn E.
dc.contributor.author Braham, Erick J.
dc.contributor.author Michienzi, Courtney L.
dc.contributor.author Flick, Robert M.
dc.contributor.author Yakunin, Alexander F.
dc.contributor.author Master, Emma R.
dc.date.accessioned 2019-01-14T09:20:32Z
dc.date.available 2019-01-14T09:20:32Z
dc.date.issued 2018-09
dc.identifier.citation Goacher , R E , Braham , E J , Michienzi , C L , Flick , R M , Yakunin , A F & Master , E R 2018 , ' Direct analysis by time-of-flight secondary ion mass spectrometry reveals action of bacterial laccase-mediator systems on both hardwood and softwood samples ' Physiologia Plantarum , vol. 164 , no. 1 , pp. 5-16 . DOI: 10.1111/ppl.12688 en
dc.identifier.issn 0031-9317
dc.identifier.other PURE UUID: 3ec7d4a0-a70f-4f58-9f8a-da3fd529c7bc
dc.identifier.other PURE ITEMURL: https://research.aalto.fi/en/publications/direct-analysis-by-timeofflight-secondary-ion-mass-spectrometry-reveals-action-of-bacterial-laccasemediator-systems-on-both-hardwood-and-softwood-samples(3ec7d4a0-a70f-4f58-9f8a-da3fd529c7bc).html
dc.identifier.other PURE LINK: http://www.scopus.com/inward/record.url?scp=85043512313&partnerID=8YFLogxK
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/35941
dc.description | openaire: EC/H2020/33947/EU//BHIVE
dc.description.abstract The modification and degradation of lignin play a vital role in carbon cycling as well as production of biofuels and bioproducts. The possibility of using bacterial laccases for the oxidation of lignin offers a route to utilize existing industrial protein expression techniques. However, bacterial laccases are most frequently studied on small model compounds that do not capture the complexity of lignocellulosic materials. This work studied the action of laccases from Bacillus subtilis and Salmonella typhimurium (EC 1.10.3.2) on ground wood samples from yellow birch (Betula alleghaniensis) and red spruce (Picea rubens). The ability of bacterial laccases to modify wood can be facilitated by small molecule mediators. Herein, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), gallic acid and sinapic acid mediators were tested. Direct analysis of the wood samples was achieved by time-of-flight secondary ion mass spectrometry (ToF-SIMS), a surface sensitive mass spectrometry technique that has characteristic peaks for H, G and S lignin. The action of the bacterial laccases on both wood samples was demonstrated and revealed a strong mediator influence. The ABTS mediator led to delignification, evident in an overall increase of polysaccharide peaks in the residual solid, along with equal loss of G and S-lignin peaks. The gallic acid mediator demonstrated minimal laccase activity. Meanwhile, the sinapic acid mediator altered the S/G peak ratio consistent with mediator attaching to the wood solids. The current investigation demonstrates the action of bacterial laccase-mediator systems directly on woody materials, and the potential of using ToF-SIMS to uncover the fundamental and applied role of bacterial enzymes in lignocellulose conversion. en
dc.format.extent 12
dc.format.extent 5-16
dc.language.iso en en
dc.relation info:eu-repo/grantAgreement/EC/H2020/33947/EU//BHIVE
dc.relation.ispartofseries Physiologia Plantarum en
dc.relation.ispartofseries Volume 164, issue 1 en
dc.rights restrictedAccess en
dc.subject.other Physiology en
dc.subject.other Genetics en
dc.subject.other Plant Science en
dc.subject.other Cell Biology en
dc.subject.other 1182 Biochemistry, cell and molecular biology en
dc.title Direct analysis by time-of-flight secondary ion mass spectrometry reveals action of bacterial laccase-mediator systems on both hardwood and softwood samples en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Niagara University
dc.contributor.department Texas A and M University
dc.contributor.department University of Toronto
dc.contributor.department Molecular Biotechnology
dc.contributor.department Department of Bioproducts and Biosystems en
dc.subject.keyword Physiology
dc.subject.keyword Genetics
dc.subject.keyword Plant Science
dc.subject.keyword Cell Biology
dc.subject.keyword 1182 Biochemistry, cell and molecular biology
dc.identifier.urn URN:NBN:fi:aalto-201901141124
dc.identifier.doi 10.1111/ppl.12688


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