Landscape of RNA polyadenylation in E. coli

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en Maes, Alexandre Gracia, Céline Innocenti, Nicolas Zhang, Kaiyang Aurell, Erik Hajnsdorf, Eliane 2018-12-21T10:31:45Z 2018-12-21T10:31:45Z 2017-03-17
dc.identifier.citation Maes , A , Gracia , C , Innocenti , N , Zhang , K , Aurell , E & Hajnsdorf , E 2017 , ' Landscape of RNA polyadenylation in E. coli ' Nucleic Acids Research , vol. 45 , no. 5 , pp. 2746-2756 . DOI: 10.1093/nar/gkw894 en
dc.identifier.issn 0305-1048
dc.identifier.issn 1362-4962
dc.identifier.other PURE UUID: 88b86c2e-05bf-4c22-98fc-80db9377328f
dc.identifier.other PURE ITEMURL:
dc.identifier.other PURE LINK:
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dc.description.abstract Polyadenylation is thought to be involved in the degradation and quality control of bacterial RNAs but relatively few examples have been investigated. We used a combination of 5'-tagRACE and RNA-seq to analyze the total RNA content from a wild-type strain and froma poly(A)polymerase deletedmutant. A total of 178 transcripts were either up- or down-regulated in the mutant when compared to the wild-type strain. Poly(A)polymerase up-regulates the expression of all genes related to the FliA regulon and several previously unknown transcripts, including numerous transporters. Notable down-regulation of genes in the expression of antigen 43 and components of the type 1 fimbriae was detected. The major consequence of the absence of poly(A)polymerase was the accumulation of numerous sRNAs, antisense transcripts, REP sequences and RNA fragments resulting from the processing of entire transcripts. A new algorithm to analyze the position and composition of post-transcriptional modifications based on the sequence of unencoded 3'-ends, was developed to identify polyadenylated molecules. Overall our results shed new light on the broad spectrum of action of polyadenylation on gene expression and demonstrate the importance of poly(A) dependent degradation to remove structured RNA fragments. en
dc.format.extent 11
dc.format.extent 2746-2756
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries Nucleic Acids Research en
dc.relation.ispartofseries Volume 45, issue 5 en
dc.rights openAccess en
dc.subject.other Genetics en
dc.subject.other 114 Physical sciences en
dc.title Landscape of RNA polyadenylation in E. coli en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Universite Paris 7
dc.contributor.department Royal Institute of Technology
dc.contributor.department University of Helsinki
dc.contributor.department Department of Applied Physics
dc.subject.keyword Genetics
dc.subject.keyword 114 Physical sciences
dc.identifier.urn URN:NBN:fi:aalto-201812216690
dc.identifier.doi 10.1093/nar/gkw894
dc.type.version publishedVersion

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