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A unique saccharomyces cerevisiae saccharomyces uvarum hybrid isolated from norwegian farmhouse beer

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Krogerus, Kristoffer
dc.contributor.author Preiss, Richard
dc.contributor.author Gibson, Brian
dc.date.accessioned 2018-12-21T10:29:44Z
dc.date.available 2018-12-21T10:29:44Z
dc.date.issued 2018-09-24
dc.identifier.citation Krogerus , K , Preiss , R & Gibson , B 2018 , ' A unique saccharomyces cerevisiae saccharomyces uvarum hybrid isolated from norwegian farmhouse beer : Characterization and reconstruction ' , Frontiers in Microbiology , vol. 9 , no. SEP , 2253 . https://doi.org/10.3389/fmicb.2018.02253 en
dc.identifier.issn 1664-302X
dc.identifier.other PURE UUID: 3c88dc24-a9aa-42c3-8117-ec827ea0c654
dc.identifier.other PURE ITEMURL: https://research.aalto.fi/en/publications/a-unique-saccharomyces-cerevisiae-saccharomyces-uvarum-hybrid-isolated-from-norwegian-farmhouse-beer(3c88dc24-a9aa-42c3-8117-ec827ea0c654).html
dc.identifier.other PURE LINK: http://www.scopus.com/inward/record.url?scp=85055084697&partnerID=8YFLogxK
dc.identifier.other PURE FILEURL: https://research.aalto.fi/files/30430034/CHEM_Krogerus_et_al_Unique_Saccharomyces_cerevisiae_2018_Frontiers_in_microbiology.pdf
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/35641
dc.description.abstract An unknown interspecies Saccharomyces hybrid, "Muri," was recently isolated from a "kveik' culture, a traditional Norwegian farmhouse brewing yeast culture (Preiss et al., 2018). Here we used whole genome sequencing to reveal the strain as an allodiploid Saccharomyces cerevisiae × Saccharomyces uvarum hybrid. Phylogenetic analysis of its sub-genomes revealed that the S. cerevisiae and S. uvarum parent strains of Muri appear to be most closely related to English ale and Central European cider and wine strains, respectively. We then performed phenotypic analysis on a number of brewing-relevant traits in a range of S. cerevisiae, S. uvarum and hybrid strains closely related to the Muri hybrid. The Muri strain possesses a range of industrially desirable phenotypic properties, including broad temperature tolerance, good ethanol tolerance, and efficient carbohydrate use, therefore making it an interesting candidate for not only brewing applications, but potentially various other industrial fermentations, such as biofuel production and distilling. We identified the two S. cerevisiae and S. uvarum strains that were genetically and phenotypically most similar to the Muri hybrid, and then attempted to reconstruct the Muri hybrid by generating de novo interspecific hybrids between these two strains. The de novo hybrids were compared with the original Muri hybrid, and many appeared phenotypically more similar to Muri than either of the parent strains. This study introduces a novel approach to studying hybrid strains and strain development by combining genomic and phenotypic analysis to identify closely related parent strains for construction of de novo hybrids. en
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries Frontiers in Microbiology en
dc.relation.ispartofseries Volume 9, issue SEP en
dc.rights openAccess en
dc.subject.other Microbiology en
dc.subject.other Microbiology (medical) en
dc.subject.other 1182 Biochemistry, cell and molecular biology en
dc.title A unique saccharomyces cerevisiae saccharomyces uvarum hybrid isolated from norwegian farmhouse beer en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Department of Bioproducts and Biosystems
dc.contributor.department University of Guelph
dc.contributor.department VTT Technical Research Centre of Finland
dc.subject.keyword Beer
dc.subject.keyword Dextrin
dc.subject.keyword Genome
dc.subject.keyword Hybrid
dc.subject.keyword Kveik
dc.subject.keyword Yeast
dc.subject.keyword Microbiology
dc.subject.keyword Microbiology (medical)
dc.subject.keyword 1182 Biochemistry, cell and molecular biology
dc.identifier.urn URN:NBN:fi:aalto-201812216649
dc.identifier.doi 10.3389/fmicb.2018.02253
dc.type.version publishedVersion

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