RNA Polymerase III Subunit POLR3G Regulates Specific Subsets of PolyA+ and SmallRNA Transcriptomes and Splicing in Human Pluripotent Stem Cells

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Lund, Riikka J.
dc.contributor.author Rahkonen, Nelly
dc.contributor.author Malonzo, Maia
dc.contributor.author Kauko, Leni
dc.contributor.author Emani, Maheswara Reddy
dc.contributor.author Kivinen, Virpi
dc.contributor.author Närvä, Elisa
dc.contributor.author Kemppainen, Esko
dc.contributor.author Laiho, Asta
dc.contributor.author Skottman, Heli
dc.contributor.author Hovatta, Outi
dc.contributor.author Rasool, Omid
dc.contributor.author Nykter, Matti
dc.contributor.author Lähdesmäki, Harri
dc.contributor.author Lahesmaa, Riitta
dc.date.accessioned 2018-12-21T10:28:40Z
dc.date.available 2018-12-21T10:28:40Z
dc.date.issued 2017-05-09
dc.identifier.citation Lund , R J , Rahkonen , N , Malonzo , M , Kauko , L , Emani , M R , Kivinen , V , Närvä , E , Kemppainen , E , Laiho , A , Skottman , H , Hovatta , O , Rasool , O , Nykter , M , Lähdesmäki , H & Lahesmaa , R 2017 , ' RNA Polymerase III Subunit POLR3G Regulates Specific Subsets of PolyA + and SmallRNA Transcriptomes and Splicing in Human Pluripotent Stem Cells ' Stem Cell Reports , vol. 8 , no. 5 , pp. 1442-1454 . DOI: 10.1016/j.stemcr.2017.04.016 en
dc.identifier.issn 2213-6711
dc.identifier.other PURE UUID: 1653a34a-0ff2-4878-b56d-4fc4f7f522ad
dc.identifier.other PURE ITEMURL: https://research.aalto.fi/en/publications/rna-polymerase-iii-subunit-polr3g-regulates-specific-subsets-of-polya-and-smallrna-transcriptomes-and-splicing-in-human-pluripotent-stem-cells(1653a34a-0ff2-4878-b56d-4fc4f7f522ad).html
dc.identifier.other PURE LINK: http://www.scopus.com/inward/record.url?scp=85019433296&partnerID=8YFLogxK
dc.identifier.other PURE FILEURL: https://research.aalto.fi/files/30359687/1_s2.0_S2213671117301704_main.pdf
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/35619
dc.description.abstract POLR3G is expressed at high levels in human pluripotent stem cells (hPSCs) and is required for maintenance of stem cell state through mechanisms not known in detail. To explore how POLR3G regulates stem cell state, we carried out deep-sequencing analysis of polyA+ and smallRNA transcriptomes present in hPSCs and regulated in POLR3G-dependent manner. Our data reveal that POLR3G regulates a specific subset of the hPSC transcriptome, including multiple transcript types, such as protein-coding genes, long intervening non-coding RNAs, microRNAs and small nucleolar RNAs, and affects RNA splicing. The primary function of POLR3G is in the maintenance rather than repression of transcription. The majority of POLR3G polyA+ transcriptome is regulated during differentiation, and the key pluripotency factors bind to the promoters of at least 30% of the POLR3G-regulated transcripts. Among the direct targets of POLR3G, POLG is potentially important in sustaining stem cell status in a POLR3G-dependent manner. en
dc.format.extent 13
dc.format.extent 1442-1454
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries Stem Cell Reports en
dc.relation.ispartofseries Volume 8, issue 5 en
dc.rights openAccess en
dc.subject.other Biochemistry en
dc.subject.other Genetics en
dc.subject.other Developmental Biology en
dc.subject.other Cell Biology en
dc.subject.other 113 Computer and information sciences en
dc.title RNA Polymerase III Subunit POLR3G Regulates Specific Subsets of PolyA+ and SmallRNA Transcriptomes and Splicing in Human Pluripotent Stem Cells en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Åbo Akademi University
dc.contributor.department Department of Computer Science
dc.contributor.department Tampere University
dc.contributor.department Karolinska Institutet
dc.subject.keyword human embryonic stem cell
dc.subject.keyword mitochondria
dc.subject.keyword pluripotency
dc.subject.keyword polyA RNA
dc.subject.keyword polymerase III
dc.subject.keyword smallRNA
dc.subject.keyword splicing
dc.subject.keyword transcriptome
dc.subject.keyword Biochemistry
dc.subject.keyword Genetics
dc.subject.keyword Developmental Biology
dc.subject.keyword Cell Biology
dc.subject.keyword 113 Computer and information sciences
dc.identifier.urn URN:NBN:fi:aalto-201812216627
dc.identifier.doi 10.1016/j.stemcr.2017.04.016
dc.type.version publishedVersion

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