An Evaluation of Boar Spermatozoa as a Biosensor for the Detection of Sublethal and Lethal Toxicity

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en Castagnoli, Emmanuelle Salo, Johanna Toivonen, Matti S. Marik, Tamás Mikkola, Raimo Kredics, László Vicente-Carrillo, Alejandro Nagy, Szabolcs Andersson, Markus T. Andersson, M. A. Kurnitski, Jarek Salonen, Heidi 2018-12-10T10:27:23Z 2018-12-10T10:27:23Z 2018-11-08
dc.identifier.citation Castagnoli , E , Salo , J , Toivonen , M S , Marik , T , Mikkola , R , Kredics , L , Vicente-Carrillo , A , Nagy , S , Andersson , M T , Andersson , M A , Kurnitski , J & Salonen , H 2018 , ' An Evaluation of Boar Spermatozoa as a Biosensor for the Detection of Sublethal and Lethal Toxicity ' Toxins , vol. 10 , no. 11 , 463 . DOI: 10.3390/toxins10110463 en
dc.identifier.issn 2072-6651
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dc.identifier.other PURE ITEMURL:
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dc.description.abstract A novel, objective, and rapid computed motility inhibition (CMI) assay was developed to identify and assess sublethal injury in toxin-exposed boar spermatozoa and compared with a subjective visual motility inhibition (VMI) assay. The CMI values were calculated from digital micrographic videos using a custom MATLAB® script by contrasting the motility index values of each experiment with those of the background and control experiments. Following a comparison of the CMI and VMI assays results, it was determined that their agreement depended on the shape of the dose-response curve. Toxins that exhibited a steep slope were indicative of good agreement between the assays. Those depicted by a gentle decline in the slope of the dose-response curve, the CMI assay were shown to be two times more sensitive than the VMI assay. The CMI assay was highly sensitive to the inhibition of mitochondrial function and glucose transport activity by sublethal doses of toxins and to disruption of cellular cation homeostasis by carrier ionophoric toxins, when compared to the cytotoxicity and lethal toxicity assays (i.e., that evaluated the inhibition of cell proliferation in somatic cell lines (FL, PK-15, and MNA cells)) and disruption to spermatozoa membrane integrity. The CMI assay recognized subtle sublethal toxicity changes in metabolism, manifested as a decrease in boar spermatozoa motility. Thus, it was feasible to effectively compare the objectively-measured numerical values for motility inhibition using the CMI assay against those reflecting lethal damage in the spermatozoa cells and somatic cell lines using a cytotoxicity assay. en
dc.format.extent 15
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries Toxins en
dc.relation.ispartofseries Volume 10, issue 11 en
dc.rights openAccess en
dc.subject.other Toxicology en
dc.subject.other Health, Toxicology and Mutagenesis en
dc.subject.other 520 Other social sciences en
dc.title An Evaluation of Boar Spermatozoa as a Biosensor for the Detection of Sublethal and Lethal Toxicity en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Department of Civil Engineering
dc.contributor.department Department of Civil and Structural Engineering
dc.contributor.department Department of Applied Physics
dc.contributor.department University of Szeged
dc.contributor.department Evidensia Valla Djursjukhus Linköping
dc.contributor.department University of Pannonia
dc.subject.keyword biological toxins
dc.subject.keyword biosensor
dc.subject.keyword boar spermatozoa
dc.subject.keyword CASA
dc.subject.keyword computed motility inhibition
dc.subject.keyword MATLAB
dc.subject.keyword toxicity
dc.subject.keyword Toxicology
dc.subject.keyword Health, Toxicology and Mutagenesis
dc.subject.keyword 520 Other social sciences
dc.identifier.urn URN:NBN:fi:aalto-201812106235
dc.identifier.doi 10.3390/toxins10110463
dc.type.version publishedVersion

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