Quantifying the interactions between biomimetic biomaterials – collagen I, collagen IV, laminin 521 and cellulose nanofibrils – by colloidal probe microscopy

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Nugroho, Robertus Wahyu N.
dc.contributor.author Harjumäki, Riina
dc.contributor.author Zhang, Xue
dc.contributor.author Lou, Yan Ru
dc.contributor.author Yliperttula, Marjo
dc.contributor.author Valle-Delgado, Juan José
dc.contributor.author Österberg, Monika
dc.date.accessioned 2018-11-09T13:05:02Z
dc.date.available 2018-11-09T13:05:02Z
dc.date.issued 2019-01-01
dc.identifier.citation Nugroho , R W N , Harjumäki , R , Zhang , X , Lou , Y R , Yliperttula , M , Valle-Delgado , J J & Österberg , M 2019 , ' Quantifying the interactions between biomimetic biomaterials – collagen I, collagen IV, laminin 521 and cellulose nanofibrils – by colloidal probe microscopy ' Colloids and Surfaces B: Biointerfaces , vol 173 , pp. 571-580 . DOI: 10.1016/j.colsurfb.2018.09.073 en
dc.identifier.issn 0927-7765
dc.identifier.issn 1873-4367
dc.identifier.other PURE UUID: 28368293-9d0b-4ab9-9167-52e745b54595
dc.identifier.other PURE ITEMURL: https://research.aalto.fi/en/publications/quantifying-the-interactions-between-biomimetic-biomaterials--collagen-i-collagen-iv-laminin-521-and-cellulose-nanofibrils--by-colloidal-probe-microscopy(28368293-9d0b-4ab9-9167-52e745b54595).html
dc.identifier.other PURE LINK: http://www.scopus.com/inward/record.url?scp=85055033323&partnerID=8YFLogxK
dc.identifier.other PURE FILEURL: https://research.aalto.fi/files/32536046/CHEM_Nugroho_et_al_Quantifying_the_interactions_2019_Colloids_and_Surfaces_B.pdf
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/34587
dc.description.abstract Biomaterials of different nature have been and are widely studied for various biomedical applications. In many cases, biomaterial assemblies are designed to mimic biological systems. Although biomaterials have been thoroughly characterized in many aspects, not much quantitative information on the molecular level interactions between different biomaterials is available. That information is very important, on the one hand, to understand the properties of biological systems and, on the other hand, to develop new composite biomaterials for special applications. This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering. Direct surface forces and adhesion between biomaterials-coated spherical microparticles and flat substrates were measured in phosphate-buffered saline using an atomic force microscope and the colloidal probe technique. Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films. The adhesion between biomaterials films increased with the time that the films were kept in contact. The strongest adhesion was observed between Col IV films, and between Col IV and LN-521 films after 30 s contact time. In contrast, low adhesion was measured between CNF films, as well as between CNF and LN-521 films. Nevertheless, a good adhesion between CNF and collagen films (especially Col I) was observed. These results increase our understanding of the structure of biological systems and can support the design of new matrices or scaffolds where different biomaterials are combined for diverse biological or medical applications. en
dc.format.extent 10
dc.format.extent 571-580
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries Colloids and Surfaces B: Biointerfaces en
dc.relation.ispartofseries Volume 173 en
dc.rights openAccess en
dc.subject.other Biotechnology en
dc.subject.other Surfaces and Interfaces en
dc.subject.other Physical and Theoretical Chemistry en
dc.subject.other Colloid and Surface Chemistry en
dc.subject.other 221 Nanotechnology en
dc.subject.other biomaterials en
dc.title Quantifying the interactions between biomimetic biomaterials – collagen I, collagen IV, laminin 521 and cellulose nanofibrils – by colloidal probe microscopy en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Department of Bioproducts and Biosystems
dc.contributor.department University of Helsinki
dc.subject.keyword Adhesion
dc.subject.keyword AFM-colloidal probe technique
dc.subject.keyword Cellulose nanofibrils
dc.subject.keyword Collagen
dc.subject.keyword Laminin
dc.subject.keyword Surface forces
dc.subject.keyword MODEL
dc.subject.keyword PROTEOGLYCAN
dc.subject.keyword MECHANICAL-PROPERTIES
dc.subject.keyword ATOMIC-FORCE MICROSCOPE
dc.subject.keyword HYDROGEL
dc.subject.keyword MOLECULES
dc.subject.keyword ORGANIZATION
dc.subject.keyword FILMS
dc.subject.keyword MICROENVIRONMENT
dc.subject.keyword CELL
dc.subject.keyword Biotechnology
dc.subject.keyword Surfaces and Interfaces
dc.subject.keyword Physical and Theoretical Chemistry
dc.subject.keyword Colloid and Surface Chemistry
dc.subject.keyword 221 Nanotechnology
dc.subject.keyword biomaterials
dc.identifier.urn URN:NBN:fi:aalto-201811095628
dc.identifier.doi 10.1016/j.colsurfb.2018.09.073

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