Interactions between Scots pine heartwood extractives and wood decaying fungi

 |  Login

Show simple item record

dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.advisor Hänninen, Tuomas, Dr., Aalto University, Department of Bioproducts and Biosystems, Finland Belt, Tiina 2018-11-07T10:03:19Z 2018-11-07T10:03:19Z 2018
dc.identifier.isbn 978-952-60-8300-1 (electronic)
dc.identifier.isbn 978-952-60-8299-8 (printed)
dc.identifier.issn 1799-4942 (electronic)
dc.identifier.issn 1799-4934 (printed)
dc.identifier.issn 1799-4934 (ISSN-L)
dc.description.abstract The heartwoods of many wood species have natural resistance to degradation caused by wood decaying fungi. Many factors can contribute to the natural durability of heartwoods, but the most significant one is usually the presence of biologically active extractives in the wood material. This thesis investigated the interactions between heartwood extractives and wood decaying fungi in detail, using Scots pine as the wood material. The primary objectives of the thesis were to study the mechanisms of action of extractives and to explore the ways in which the extractives interact with fungi within the structure of wood. The mechanism of action studies focused on the antioxidant activity of extractives and on their ability to inhibit the enzymatic hydrolysis of wood polysaccharides. The antioxidant activity measurements showed that Scots pine heartwood extractives are active antioxidants, which means that they may be able to interfere with the radical-based degradative mechanisms used by wood decaying fungi, particularly brown rots. The extractives could also inhibit the action of hydrolytic enzymes, suggesting that they may be able to prevent the conversion of wood polysaccharides to digestible sugars. However, substantial hydrolase inhibition was only seen with a white rot enzyme preparation, which contained some enzyme(s) capable of modifying the heartwood extractives. The interaction studies used confocal Raman spectroscopy imaging to visualise the cellular level distributions of extractives and other chemical components in intact and decaying heartwood. The studies revealed that the phenolic pinosylvins were present throughout the heartwood tissues, suggesting that they have good ability to interact with fungi and their degradative agents during decay. However, the studies on decaying heartwood showed that pinosylvins were extensively degraded during incipient decay. The hydrophobic resin acids were only detected in the lumens of some tracheids and ray cells, but they were found to have higher resistance to degradation than the pinosylvins. Interestingly, the resin-rich extractives deposits found in tracheid lumens appeared to create local areas of reduced degradation in the decaying heartwood. The results of this thesis provide new information on the ways in which extractives can interact with fungi and contribute to natural durability. The results also increase our understanding of the origins of natural durability in Scots pine, the most abundant wood species in Finland. en
dc.format.extent 80 + app. 54
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Aalto University en
dc.publisher Aalto-yliopisto fi
dc.relation.ispartofseries Aalto University publication series DOCTORAL DISSERTATIONS en
dc.relation.ispartofseries 227/2018
dc.relation.haspart [Publication 1]: Belt T, Hänninen T, Rautkari L. Antioxidant activity of Scots pine heartwood and knot extractives and implications for resistance to brown rot. Holzforschung, 2017, 71:527–534. DOI: 10.1515/hf-2016-0232
dc.relation.haspart [Publication 2]: Belt T, Mollerup F, Hänninen T, Rautkari L. Inhibitory effects of Scots pine heartwood extractives on enzymatic holocellulose hydrolysis by wood decaying fungi. International Biodeterioration & Biodegradation, 2018, 132:150-156. DOI: 10.1016/j.ibiod.2018.03.004
dc.relation.haspart [Publication 3]: Belt T, Keplinger T, Hänninen T, Rautkari L. Cellular level distributions of Scots pine heartwood and knot heartwood extractives revealed by Raman spectroscopy imaging. Industrial Crops & Products, 2017, 108:327–335. Full Text in Acris/Aaltodoc: DOI: 10.1016/j.indcrop.2017.06.056
dc.relation.haspart [Publication 4]: Belt T, Altgen M, Mäkelä M, Hänninen T, Rautkari L. Cellular level chemical changes in Scots pine heartwood during incipient brown rot decay. 2018. Submitted to Scientific Reports.
dc.subject.other Biotechnology en
dc.subject.other Chemistry en
dc.title Interactions between Scots pine heartwood extractives and wood decaying fungi en
dc.type G5 Artikkeliväitöskirja fi Kemian tekniikan korkeakoulu fi School of Chemical Technology en
dc.contributor.department Biotuotteiden ja biotekniikan laitos fi
dc.contributor.department Department of Bioproducts and Biosystems en
dc.subject.keyword extractives en
dc.subject.keyword heartwood en
dc.subject.keyword pinosylvin en
dc.subject.keyword Raman imaging en
dc.subject.keyword resin acid en
dc.subject.keyword wood decay en
dc.identifier.urn URN:ISBN:978-952-60-8300-1
dc.type.dcmitype text en
dc.type.ontasot Doctoral dissertation (article-based) en
dc.type.ontasot Väitöskirja (artikkeli) fi
dc.contributor.supervisor Rautkari, Lauri, Prof., Aalto University, Department of Bioproducts and Biosystems, Finland
dc.opn Burgert, Ingo, Prof., ETH Zürich, Switzerland
dc.contributor.lab Wood material science and technology en
dc.rev Morrell, Jeffrey, Prof., Oregon State University, USA
dc.rev Gierlinger Notburga, Prof., University of Natural Resources and Life Sciences, Austria 2018-11-23
local.aalto.acrisexportstatus checked 2019-02-19_1537

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search archive

Advanced Search

article-iconSubmit a publication


My Account