Determination of the Main Phase Transition Temperature of Phospholipids by Nanoplasmonic Sensing

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en Chen, Wen Duša, Filip Witos, Joanna Ruokonen, Suvi Katriina Wiedmer, Susanne K. 2018-10-24T09:41:07Z 2018-10-24T09:41:07Z 2018-12-01
dc.identifier.citation Chen , W , Duša , F , Witos , J , Ruokonen , S K & Wiedmer , S K 2018 , ' Determination of the Main Phase Transition Temperature of Phospholipids by Nanoplasmonic Sensing ' Scientific Reports , vol 8 , no. 1 , 14815 . DOI: 10.1038/s41598-018-33107-5 en
dc.identifier.issn 2045-2322
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dc.description.abstract Our study demonstrates that nanoplasmonic sensing (NPS) can be utilized for the determination of the phase transition temperature (Tm) of phospholipids. During the phase transition, the lipid bilayer undergoes a conformational change. Therefore, it is presumed that the Tm of phospholipids can be determined by detecting conformational changes in liposomes. The studied lipids included 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). Liposomes in gel phase are immobilized onto silicon dioxide sensors and the sensor cell temperature is increased until passing the Tm of the lipid. The results show that, when the system temperature approaches the Tm, a drop of the NPS signal is observed. The breakpoints in the temperatures are 22.5 °C, 41.0 °C, and 55.5 °C for DMPC, DPPC, and DSPC, respectively. These values are very close to the theoretical Tm values, i.e., 24 °C, 41.4 °C, and 55 °C for DMPC, DPPC, and DSPC, respectively. Our studies prove that the NPS methodology is a simple and valuable tool for the determination of the Tm of phospholipids. en
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries Scientific Reports en
dc.relation.ispartofseries Volume 8, issue 1 en
dc.rights openAccess en
dc.subject.other General en
dc.subject.other 221 Nanotechnology en
dc.title Determination of the Main Phase Transition Temperature of Phospholipids by Nanoplasmonic Sensing en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department University of Helsinki
dc.contributor.department Czech Academy of Sciences
dc.contributor.department Department of Bioproducts and Biosystems
dc.subject.keyword General
dc.subject.keyword 221 Nanotechnology
dc.identifier.urn URN:NBN:fi:aalto-201810245547
dc.identifier.doi 10.1038/s41598-018-33107-5
dc.type.version publishedVersion

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