Production of α-1,3-L-arabinofuranosidase active on substituted xylan does not improve compost degradation by Agaricus bisporus

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Vos, Aurin M.
dc.contributor.author Jurak, Edita
dc.contributor.author de Gijsel, Peter
dc.contributor.author Ohm, Robin A.
dc.contributor.author Henrissat, Bernard
dc.contributor.author Lugones, Luis G.
dc.contributor.author Kabel, Mirjam A.
dc.contributor.author Wösten, Han A.B.
dc.date.accessioned 2018-10-16T08:54:12Z
dc.date.available 2018-10-16T08:54:12Z
dc.date.issued 2018-07-01
dc.identifier.citation Vos , A M , Jurak , E , de Gijsel , P , Ohm , R A , Henrissat , B , Lugones , L G , Kabel , M A & Wösten , H A B 2018 , ' Production of α-1,3-L-arabinofuranosidase active on substituted xylan does not improve compost degradation by Agaricus bisporus ' PLOS ONE , vol 13 , no. 7 , e0201090 . DOI: 10.1371/journal.pone.0201090 en
dc.identifier.issn 1932-6203
dc.identifier.other PURE UUID: 4733cff9-2dd9-442a-ba84-1833a84f79d1
dc.identifier.other PURE ITEMURL: https://research.aalto.fi/en/publications/production-of-13larabinofuranosidase-active-on-substituted-xylan-does-not-improve-compost-degradation-by-agaricus-bisporus(4733cff9-2dd9-442a-ba84-1833a84f79d1).html
dc.identifier.other PURE LINK: http://www.scopus.com/inward/record.url?scp=85050403648&partnerID=8YFLogxK
dc.identifier.other PURE FILEURL: https://research.aalto.fi/files/28320095/CHEM_Vos_et_2018_PlosOne_e0201090.pdf
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/34272
dc.description.abstract Agaricus bisporus consumes carbohydrates contained in wheat straw based compost used for commercial mushroom production. Double substituted arabinoxylan is part of the ~40% of the compost polysaccharides that are not degraded by A. bisporus during its growth and development. Genes encoding α-1,3-L-arabinofuranosidase (AXHd3) enzymes that act on xylosyl residues doubly substituted with arabinosyl residues are absent in this mushroom forming fungus. Here, the AXHd3 encoding hgh43 gene of Humicola insolens was expressed in A. bisporus with the aim to improve its substrate utilization and mushroom yield. Transformants secreted active AXHd3 in compost as shown by the degradation of double substituted arabinoxylan oligomers in an in vitro assay. However, carbohydrate composition and degree of arabinosyl substitution of arabinoxylans were not affected in compost possibly due to inaccessibility of the doubly substituted xylosyl residues. en
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries PLOS ONE en
dc.relation.ispartofseries Volume 13, issue 7 en
dc.rights openAccess en
dc.subject.other Biochemistry, Genetics and Molecular Biology(all) en
dc.subject.other Agricultural and Biological Sciences(all) en
dc.subject.other 1182 Biochemistry, cell and molecular biology en
dc.title Production of α-1,3-L-arabinofuranosidase active on substituted xylan does not improve compost degradation by Agaricus bisporus en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Utrecht University
dc.contributor.department Department of Bioproducts and Biosystems
dc.contributor.department Wageningen University & Research
dc.contributor.department Aix-Marseille Université
dc.subject.keyword Biochemistry, Genetics and Molecular Biology(all)
dc.subject.keyword Agricultural and Biological Sciences(all)
dc.subject.keyword 1182 Biochemistry, cell and molecular biology
dc.identifier.urn URN:NBN:fi:aalto-201810165349
dc.identifier.doi 10.1371/journal.pone.0201090
dc.type.version publishedVersion


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