In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography
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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Date
2018-08
Major/Subject
Mcode
Degree programme
Language
en
Pages
1118-1126
Series
Osteoarthritis and Cartilage, Volume 26, issue 8
Abstract
Objective: The aims of this study were: to 1) develop a novel sample processing protocol to visualize human articular cartilage (AC) chondrons using micro-computed tomography (μCT), 2) develop and validate an algorithm to quantify the chondron morphology in 3D, and 3) compare the differences in chondron morphology between intact and osteoarthritic AC. Method: The developed protocol is based on the dehydration of samples with hexamethyldisilazane (HMDS), followed by imaging with a desktop μCT. Chondron density and depth, as well as volume and sphericity, were calculated in 3D with a custom-made and validated algorithm employing semi-automatic chondron selection and segmentation. The quantitative parameters were analyzed at three AC depth zones (zone 1: 0–10%; zone 2: 10–40%; zone 3: 40–100%) and grouped by the OARSI histological grades (OARSI grades 0–1.0, n = 6; OARSI grades 3.0–3.5, n = 6). Results: After semi-automatic chondron selection and segmentation, 1510 chondrons were approved for 3D morphometric analyses. The chondrons especially in the deeper tissue (zones 2 and 3) were significantly larger (P < 0.001) and less spherical (P < 0.001), respectively, in the OARSI grade 3–3.5 group compared to the OARSI grade 0–1.0 group. No statistically significant difference in chondron density between the OARSI grade groups was observed at different depths. Conclusion: We have developed a novel sample processing protocol for chondron imaging in 3D, as well as a high-throughput algorithm to semi-automatically quantify chondron/chondrocyte 3D morphology in AC. Our results also suggest that 3D chondron morphology is affected by the progression of osteoarthritis (OA).Description
Keywords
3D analysis, Hexamethyldisilazane, Morphology, Osteoarthritis, Segmentation
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Citation
Kestilä , I , Thevenot , J , Finnilä , M A , Karhula , S S , Hadjab , I , Kauppinen , S , Garon , M , Quenneville , E , Haapea , M , Rieppo , L , Pritzker , K P , Buschmann , M D , Nieminen , H J & Saarakkala , S 2018 , ' In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography ' , Osteoarthritis and Cartilage , vol. 26 , no. 8 , pp. 1118-1126 . https://doi.org/10.1016/j.joca.2018.05.012
