Phase transitions as intermediate steps in the formation of molecularly engineered protein fibers

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Mohammadi, Pezhman
dc.contributor.author Aranko, Aino Sesilja
dc.contributor.author Lemetti, Laura
dc.contributor.author Cenev, Zoran
dc.contributor.author Zhou, Quan
dc.contributor.author Virtanen, Salla
dc.contributor.author Landowski, Christopher P.
dc.contributor.author Penttilä, Merja
dc.contributor.author Fischer, Wolfgang J.
dc.contributor.author Wagermaier, Wolfgang
dc.contributor.author Linder, Markus
dc.date.accessioned 2018-09-21T09:49:24Z
dc.date.available 2018-09-21T09:49:24Z
dc.date.issued 2018
dc.identifier.citation Mohammadi , P , Aranko , A S , Lemetti , L , Cenev , Z , Zhou , Q , Virtanen , S , Landowski , C P , Penttilä , M , Fischer , W J , Wagermaier , W & Linder , M 2018 , ' Phase transitions as intermediate steps in the formation of molecularly engineered protein fibers ' Communications Biology , vol 1 , 86 . DOI: 10.1038/s42003-018-0090-y en
dc.identifier.issn 2399-3642
dc.identifier.other PURE UUID: 6bdd352c-3f7c-47e6-9082-ce8a57adbc87
dc.identifier.other PURE ITEMURL: https://research.aalto.fi/en/publications/phase-transitions-as-intermediate-steps-in-the-formation-of-molecularly-engineered-protein-fibers(6bdd352c-3f7c-47e6-9082-ce8a57adbc87).html
dc.identifier.other PURE FILEURL: https://research.aalto.fi/files/27944523/Mohammadi_et_al_2018_Communications_Biology.pdf
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/34053
dc.description.abstract A central concept in molecular bioscience is how structure formation at different length scales is achieved. Here we use spider silk protein as a model to design new recombinant proteins that assemble into fibers. We made proteins with a three-block architecture with folded globular domains at each terminus of a truncated repetitive silk sequence. Aqueous solutions of these engineered proteins undergo liquid–liquid phase separation as an essential pre-assembly step before fibers can form by drawing in air. We show that two different forms of phase separation occur depending on solution conditions, but only one form leads to fiber assembly. Structural variants with one-block or two block architectures do not lead to fibers. Fibers show strong adhesion to surfaces and self-fusing properties when placed into contact with each other. Our results show a link between protein architecture and phase separation behavior suggesting a general approach for understanding protein assembly from dilute solutions into functional structures. en
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries Communications Biology en
dc.relation.ispartofseries Volume 1 en
dc.rights openAccess en
dc.subject.other 1182 Biochemistry, cell and molecular biology en
dc.title Phase transitions as intermediate steps in the formation of molecularly engineered protein fibers en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Biomolecular materials
dc.contributor.department Department of Electrical Engineering and Automation
dc.contributor.department Department of Bioproducts and Biosystems
dc.contributor.department VTT Technical Research Centre of Finland
dc.contributor.department Sappi Papier Holding GmbH
dc.contributor.department Max Planck Institute of Colloids and Interfaces
dc.subject.keyword 1182 Biochemistry, cell and molecular biology
dc.identifier.urn URN:NBN:fi:aalto-201809215148
dc.identifier.doi 10.1038/s42003-018-0090-y
dc.type.version publishedVersion


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