Coacervation of resilin fusion proteins containing terminal functionalities

Fang, Wenwen; Nonappa; Vitikainen, Marika; Mohammadi, Pezhman; Koskela, Salla; Soikkeli, Miika; Westerholm-Parvinen, Ann; Landowski, Christopher P.; Penttilä, Merja; Linder, Markus B.; Laaksonen, Päivi

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Coacervation of resilin fusion proteins containing terminal functionalities

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Title:	Coacervation of resilin fusion proteins containing terminal functionalities
Author(s):	Fang, Wenwen ; Nonappa ; Vitikainen, Marika ; Mohammadi, Pezhman ; Koskela, Salla ; Soikkeli, Miika ; Westerholm-Parvinen, Ann ; Landowski, Christopher P. ; Penttilä, Merja ; Linder, Markus B. ; Laaksonen, Päivi
Date:	2018-11-01
Language:	en
Pages:	7 590-596
Department:	Department of Bioproducts and Biosystems VTT Technical Research Centre of Finland Department of Applied Physics
Series:	Colloids and Surfaces B: Biointerfaces, Volume 171
ISSN:	0927-7765 1873-4367
DOI-number:	10.1016/j.colsurfb.2018.07.048
Keywords:	Resilin, Self-assembly, Coacervation, Selective adhesion, Tomography, TROPOELASTIN, HYDROPHOBINS, CELLULOSE, REC1-RESILIN, GRAPHENE
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Citation: Fang , W , Nonappa , Vitikainen , M , Mohammadi , P , Koskela , S , Soikkeli , M , Westerholm-Parvinen , A , Landowski , C P , Penttilä , M , Linder , M B & Laaksonen , P 2018 , ' Coacervation of resilin fusion proteins containing terminal functionalities ' , Colloids and Surfaces B: Biointerfaces , vol. 171 , pp. 590-596 . https://doi.org/10.1016/j.colsurfb.2018.07.048
Abstract: Liquid-liquid phase transition known as coacervation of resilin-like-peptide fusion proteins containing different terminal domains were investigated. Two different modular proteins were designed and produced and their behavior were compared to a resilin-like-peptide without terminal domains. The size of the particle-like coacervates was modulated by the protein concentration, pH and temperature. The morphology and three-dimensional (3D) structural details of the coacervate particles were investigated by cryogenic transmission electron microscopy (cryo-TEM) and tomography (cryo-ET) reconstruction. Selective adhesion of the coacervates on cellulose and graphene surfaces was demonstrated.

Permanent link to this item: http://urn.fi/URN:NBN:fi:aalto-201809115113 Email this Export to RefWorks QR Code Print BibTex Tweet