Fractionation process for the protective isolation of ergosterol and trehalose from microbial biomass

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en Pastinen, Ossi Nyyssölä, Antti Pihlajaniemi, Ville Sipponen, Mika Henrikki 2018-02-09T10:01:32Z 2018-02-09T10:01:32Z 2017-07
dc.identifier.citation Pastinen , O , Nyyssölä , A , Pihlajaniemi , V & Sipponen , M H 2017 , ' Fractionation process for the protective isolation of ergosterol and trehalose from microbial biomass ' Process Biochemistry , vol 58 , pp. 217-223 . DOI: 10.1016/j.procbio.2017.04.002 en
dc.identifier.issn 1359-5113
dc.identifier.other PURE UUID: 8db830fd-33fd-437a-9f79-c3818edb9fff
dc.identifier.other PURE ITEMURL:
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dc.description.abstract A new process is described for the two phase extraction of ergosterol and trehalose from microbial biomass. Baker's yeast was used as a model organism to develop the method, which was then applied for extracting 13 oleaginous microbes. Major findings of the study were that the ergosterol content was not dependent on intracellular oil content and that 1-butanol and alkaline pH were needed to protect ergosterol. Saponification for 3-4. h at 85-100. °C followed by extraction of the reaction mixture with toluene gave the maximal ergosterol yield. Trehalose was stable at this temperature and remained in water solution, but the maximal yield was obtained after a shorter reaction time at lower alkalinity. Although trehalose alone is stable at alkaline pH, extraction yields of trehalose from yeast decreased with increasing alkalinity. This finding led us to propose a two-step process in which trehalose is separated in the first step and ergosterol in the second. The possibility to apply this method to fractionate oleaginous microbes in process scale is discussed from technical viewpoints. en
dc.format.extent 6
dc.format.extent 217-223
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries Process Biochemistry en
dc.relation.ispartofseries Volume 58 en
dc.rights openAccess en
dc.subject.other Bioengineering en
dc.subject.other Biochemistry en
dc.subject.other Applied Microbiology and Biotechnology en
dc.subject.other 215 Chemical engineering en
dc.subject.other 1182 Biochemistry, cell and molecular biology en
dc.title Fractionation process for the protective isolation of ergosterol and trehalose from microbial biomass en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Department of Chemical and Metallurgical Engineering
dc.contributor.department VTT Technical Research Centre of Finland
dc.contributor.department Department of Bioproducts and Biosystems
dc.subject.keyword Ergosterol trehalose
dc.subject.keyword Extraction
dc.subject.keyword Fractionation
dc.subject.keyword Microbial oil
dc.subject.keyword Yeast
dc.subject.keyword Bioengineering
dc.subject.keyword Biochemistry
dc.subject.keyword Applied Microbiology and Biotechnology
dc.subject.keyword 215 Chemical engineering
dc.subject.keyword 1182 Biochemistry, cell and molecular biology
dc.identifier.urn URN:NBN:fi:aalto-201802091397
dc.identifier.doi 10.1016/j.procbio.2017.04.002 info:eu-repo/date/embargoEnd/2018-08-01

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