Applications of electrochemiluminescence detection on microfabricated devices

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en Spehar-Délèze, Anna-Maria 2012-02-24T07:40:37Z 2012-02-24T07:40:37Z 2006-10-06
dc.identifier.isbn 951-22-8384-0
dc.identifier.issn 1795-4584
dc.description.abstract The aim of this thesis was to investigate bioanalytical applications of electrochemiluminescence (ECL), which refers to the generation of light at the surface of an electrode. Two types of ECL detection were studied: anodic ECL and cathodic hot electron-induced ECL (HECL). In anodic ECL light is generated at traditional electrode materials, such as noble metal or carbon, while in cathodic HECL thin insulating film-coated electrodes are used, and light generation is initiated by tunnel emission of hot, energetic electrons. Both types of ECL provide high spatial control. ECL applications for hybridization assays were investigated. Short 15-base oligonucleotide probes were immobilized on gold and oxide-coated silicon and aluminum electrodes. Hybridization with complementary targets was detected by ECL. Results showed that the oligonucleotides were successfully immobilized and high surface probe density was achieved. Labeled targets were detected at subnanomolar concentration levels. Two base pair mismatches were successfully discriminated. A homogeneous hybridization assay where hybridization was detected by quenching of anodic ECL of a Ru(bpy)32+ label by another luminophore (Cy5) was performed on thin film carbon electrodes. The quenching efficiency was 78% when the distance between the label moieties was short (≤ 2 nm). Also, an immunoassay on double barrier aluminum/aluminum oxide electrodes with Tb(III) chelate as the HECL label was performed. A microfluidic system was fabricated in poly(dimethylsiloxane) (PDMS) and glass with integrated carbon fiber and platinum electrodes, and tested for direct ECL detection of guanosine. The magnitude of electroosmotic flow (EOF) in PDMS microchannels was determined using the current monitoring method. Results revealed that the origin of the surface charge in PDMS is the same as in silica, but its amount is considerably lower. en
dc.format.extent 150
dc.format.mimetype application/pdf
dc.language.iso en en
dc.publisher Helsinki University of Technology en
dc.publisher Teknillinen korkeakoulu fi
dc.relation.ispartofseries TKK dissertations en
dc.relation.ispartofseries 44 en
dc.subject.other Chemistry en
dc.title Applications of electrochemiluminescence detection on microfabricated devices en
dc.type G4 Monografiaväitöskirja fi
dc.description.version reviewed en
dc.contributor.department Department of Chemical Technology en
dc.contributor.department Kemian tekniikan osasto fi
dc.subject.keyword electrochemiluminescence en
dc.subject.keyword hot electron en
dc.subject.keyword DNA hybridization en
dc.subject.keyword immunoassay en
dc.subject.keyword microfabrication en
dc.identifier.urn urn:nbn:fi:tkk-008170
dc.type.dcmitype text en
dc.type.ontasot Väitöskirja (monografia) fi
dc.type.ontasot Doctoral dissertation (monograph) en
dc.contributor.lab Laboratory of Inorganic and Analytical Chemistry en
dc.contributor.lab Epäorgaanisen ja analyyttisen kemian laboratorio fi

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