Biocompatibility of Liposome Nanocarriers in the Rat Inner Ear After Intratympanic Administration

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Zou, Jing
dc.contributor.author Feng, Hao
dc.contributor.author Sood, Rohit
dc.contributor.author Kinnunen, Paavo K.J.
dc.contributor.author Pyykko, Ilmari
dc.date.accessioned 2017-07-05T05:55:19Z
dc.date.available 2017-07-05T05:55:19Z
dc.date.issued 2017
dc.identifier.citation Zou , J , Feng , H , Sood , R , Kinnunen , P K J & Pyykko , I 2017 , ' Biocompatibility of Liposome Nanocarriers in the Rat Inner Ear After Intratympanic Administration ' NANOSCALE RESEARCH LETTERS , vol 12 , 372 , pp. 1-14 . DOI: 10.1186/s11671-017-2142-5 en
dc.identifier.issn 1931-7573
dc.identifier.issn 1556-276X
dc.identifier.other PURE UUID: 6c69965d-fe57-458b-bcc1-3fb10a0810ab
dc.identifier.other PURE ITEMURL: https://research.aalto.fi/en/publications/biocompatibility-of-liposome-nanocarriers-in-the-rat-inner-ear-after-intratympanic-administration(6c69965d-fe57-458b-bcc1-3fb10a0810ab).html
dc.identifier.other PURE LINK: http://www.scopus.com/inward/record.url?scp=85019764244&partnerID=8YFLogxK
dc.identifier.other PURE FILEURL: https://research.aalto.fi/files/13680921/redirect.pdf
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/27285
dc.description.abstract Liposome nanocarriers (LPNs) are potentially the future of inner ear therapy due to their high drug loading capacity and efficient uptake in the inner ear after a minimally invasive intratympanic administration. However, information on the biocompatibility of LPNs in the inner ear is lacking. The aim of the present study is to document the biocompatibility of LPNs in the inner ear after intratympanic delivery. LPNs with or without gadolinium-tetra-azacyclo-dodecane-tetra-acetic acid (Gd-DOTA) were delivered to the rats through transtympanic injection. The distribution of the Gd-DOTA-containing LPNs in the middle and inner ear was tracked in vivo using MRI. The function of the middle and inner ear barriers was evaluated using gadolinium-enhanced MRI. The auditory function was measured using auditory brainstem response (ABR). The potential inflammatory response was investigated by analyzing glycosaminoglycan and hyaluronic acid secretion and CD44 and TLR2 expression in the inner ear. The potential apoptosis wasanalyzed using terminal transferase (TdT) to label the free 3′OH breaks in the DNA strands of apoptotic cells with TMR-dUTP (TUNEL staining). As a result, LPNs entered the inner ear efficiently after transtympanic injection. The transtympanic injection of LPNs with or without Gd-DOTA neither disrupted the function of the middle and inner ear barriers nor caused hearing impairment in rats. The critical inflammatory biological markers in the inner ear, including glycosaminoglycan and hyaluronic acid secretion and CD44 and TLR2 expression, were not influenced by the administration of LPNs. There was no significant cell death associated with the administration of LPNs. The transtympanic injection of LPNs is safe for the inner ear, and LPNs may be applied as a drug delivery matrix in the clinical therapy of sensorineural hearing loss. en
dc.format.extent 1-14
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries NANOSCALE RESEARCH LETTERS en
dc.relation.ispartofseries Volume 12 en
dc.rights openAccess en
dc.subject.other Materials Science(all) en
dc.subject.other Condensed Matter Physics en
dc.subject.other 217 Medical engineering en
dc.title Biocompatibility of Liposome Nanocarriers in the Rat Inner Ear After Intratympanic Administration en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Second Military Medical University
dc.contributor.department University of Tampere
dc.contributor.department School services, SCI
dc.contributor.department Department of Neuroscience and Biomedical Engineering
dc.subject.keyword Animal
dc.subject.keyword Biological Response
dc.subject.keyword Drug Delivery
dc.subject.keyword Inner Ear
dc.subject.keyword Liposome
dc.subject.keyword Nanomaterial
dc.subject.keyword Materials Science(all)
dc.subject.keyword Condensed Matter Physics
dc.subject.keyword 217 Medical engineering
dc.identifier.urn URN:NBN:fi:aalto-201707056318
dc.identifier.doi 10.1186/s11671-017-2142-5
dc.type.version publishedVersion


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