| Title: | Reproducibility of fluorescent expression from engineered biological constructs in E. coli |
| Author(s): | Beal, Jacob ; Haddock-Angelli, Traci ; Gershater, Markus ; De Mora, Kim ; Lizarazo, Meagan ; Hollenhorst, Jim ; Rettberg, Randy ; Demling, Philipp ; Hanke, Rene ; Osthege, Michael ; Schechtel, Anna ; Sudarsan, Suresh ; Zimmermann, Arne ; Gabryelczyk, Bartosz ; Ikonen, Martina ; Salmela, Minnamari ; Acar, Muradýye ; Aktas, Muhammed Fatih ; Bestepe, Furkan ; Ceylan, Furkan Sacit ; Cigdem, Sadýk ; Dohan, Mikail ; Elitok, Mustafa ; Gunduz, Mehmet ; Gunduz, Esra ; Hatipoglu, Omer Faruk ; Kaya, Turan ; Sayin, Orhan ; Tapan, Safa ; Tereci, Osman Faruk ; Uçar, Abdullah ; Yilmaz, Mustafa ; Barrick, Jeffrey ; Gutierrez, Alex ; Mishler, Dennis ; Monk, Jordan ; Mortensen, Kate ; Shin, Nathan ; Watkins, Ella ; Chen, Yintong ; Jin, Yuji ; Shi, Yuanjie ; Zhang, Haoqian Myelin ; Ono, Bruno ; Paino, Ieda Maria Martinez ; Ribovski, Lais ; Silva, Ivan ; Zampronio, Danilo Keiji ; Birkholz, Nils ; Busche, Rudiger Frederik ; Konzock, Oliver ; Lippold, Steffen ; Ludwig, Carsten ; Philippi, Melanie ; Platz, Lukas ; Sigismund, Christian ; Weber, Susanne ; Wehrs, Maren ; Werchau, Niels ; Wronska, Anna ; Yen, Zen Zen ; Agarwal, Yash ; Appleton, Evan ; Densmore, Douglas ; Esmurria, Ariela ; Lewis, Kathleen ; Pacheco, Alan ; Bruchez, Marcel ; Peters, Danielle ; Telmer, Cheryl ; Wang, Lena ; Canas-Duarte, Silvia ; Giraldo-Perez, Daniel ; Gomez-Garzon, Camilo ; Madrid-Wolff, Jorge ; Marin-Medina, Nathaly ; Mazzanti, Valentina ; Rodriguez-Forero, Laura ; Scher, Eitan ; Dowell, Robin ; O'Hara, Samantha ; Pogoda, Cloe Simone ; Shattuck, Kendra ; Altintas, Ali ; Bali, Anne Pihl ; Bech, Rasmus ; Egholm, Anne ; Hansen, Anne Sofie Laerke ; Jensen, Kristian ; Karlsen, Kristian Barreth ; Mosbech, Caroline ; Belkhelfa, Sophia ; Berenger, Noemie ; Bodinier, Romain ; Jacry, Cecile ; Matabishi-Bibi, Laura ; Parutto, Pierre ; Zaworski, Julie ; De Vries, Andries ; De Wijs, Freek ; Elbert, Rick ; Hielkema, Lisa ; Jagadeesan, Chandhuru ; Jayawardhana, Bayu ; Kuipers, Oscar ; Lauxen, Anna ; Meijer, Thomas ; Mous, Sandra ; Van Raaphorst, Renske ; Saraf, Aakanksha ; Schepers, Otto ; Smits, Oscar ; Veening, Jan Willem ; Weme, Ruud Detert Oude ; Wieske, Lianne ; Ainsworth, Catherine ; Spencer-Milnes, Xenia ; Ávila, Alejandro Gómez ; Gamez, Eddie Cano ; Huerta, Ana Laura Torres ; Ramirez, Carlos Alejandro Meza ; Popp, Philipp ; Radeck, Jara ; Sommer, Anna ; Li, Xiangkai ; Wu, Qi ; Zhao, Hongxia ; Zhao, Ruixue ; Bastuzel, Irem ; Ceyhan, Yasemin ; Gursel, Mayda ; Kizil, Burak ; Kumru, Ilkem ; Kuvvet, Yasemin ; Tercan, Helin ; Yuksel, Seniz ; Niyazmetova, Luiza ; Ang, Timothy ; Black, Lucas ; Kelly, Ciaran ; Wadhams, George ; Basier, Clovis ; Czerwinska, Urszula ; Ananda, Cindy Suci ; Al Azhar, Muhammad ; Elviantari, Adelia ; Fitriana, Maya ; Witarto, Arief Budi ; Yulianti ; Hou, Qingfeng ; Pei, Wan ; Rifei, Chen ; Rong, Wang ; Wei, Huang ; Yushan, Zhang ; Jianguo, He ; Lai, Dengwen ; Li, Pai ; Liu, Jianheng ; Ni, Chunyang ; Zhang, Qianbin ; Cadenas, Cinthya ; Canabal, Eduardo J Zardain ; Cantu, Claudia Nallely Alonso ; Cantu, Mercedes Alejandra Vazquez ; Canedo, Eduardo Cepeda ; Cordova, Cesar Miguel Valdez ; De La Paz Espinosa, Jose Alberto ; Garcia, Carlos Enrique Alavez ; Heredia, Ana Laura Navarro ; Hernandez, Adriana ; Jauregui, Sebastian Valdivieso ; Montiel, Eduardo Ramirez ; Morales, Eduardo Serna ; Morales, Yamile Minerva Castellanos ; Pena, Omar Alonso Cantu ; Ramirez-Rodríguez, Eduardo A. ; Trejo, Elizabeth Vallejo ; Ceja, Jesus Gilberto Rodriguez ; Hernandez, Jesus Eduardo Martinez ; Hernandez, Mario Alberto Pena ; Perez, Enrique Amaya ; Ramirez, Rebeca Paola Torres ; Rocha, J. Claudio Moreno ; Sanchez, Alber ; Vazquez, Claudia Melissa Guerra ; Hanzel, Martin ; Mohand-Said, Sarah ; Sawar, Shihab ; Siriwardena, Dylan ; Tzahristos, Alex ; Anlind, Nils ; Friberg, Martin ; Gullberg, Erik ; Herman, Stephanie ; Christensen, Dallin ; Gertsch, Sara ; Maxfield, Cody ; Miller, Charles ; Putman, Ryan ; Bauerl, Christine ; Lopez, Lucia T Estelles ; Huet-Trujillo, Estefania ; Vilar, Marta Vazquez ; Birk, Marlène Sophie ; Claassens, Nico ; De Koster, Walter ; Van Rosmalen, Rik ; Wu, Wen Ying ; Davies, Sian ; Goss, Dan ; Rostain, William ; Tye, Chelsey ; Yousaf, Waqar ; Farny, Natalie ; LaJeunesse, Chloe ; Turland, Alex ; Chen, An ; Chen, Jielin ; Chen, Yahong ; Chen, Zehua ; Fang, Baishan ; Fu, Xiaotong ; Guo, Xifeng ; Lei, Yiying ; Li, Jianqiao ; Li, Zhe ; Liu, Chang ; Liu, Weibing ; Liu, Yang ; Lv, Yizhu ; Ruan, Qingyu ; Su, Yue ; Tang, Chun ; Wang, Yushen ; Wu, Fan ; Yan, Xiaoshan ; Zhang, Ruihua ; Zhang, Tangduo ; Isaacs, Farren ; Leyva-Hernandez, Ariel ; Ma, Natalie ; Mao, Stephanie ; Naidu, Yamini ; Miinalainen, Tuukka ; Aruanno, Marion ; Calendini, Daniel ; Chabert, Yoann ; Chambonnier, Gael ; Choukour, Myriam ; De Gaulejac, Ella ; Houy, Camille ; Levier, Axel ; Logger, Loreen ; Nin, Sebastien ; Prima, Valerie ; Sturgis, James N. ; Fang, Beibei ; Cigdem, Sadik ; Gutierrez, Alejandro ; Poondla, Revanth ; Reddy, Sanjana ; Rocha, Tyler ; Schulte, Natalie ; Wehle, Devin ; Jackowski, Marta Eva ; Craig, Sean Ross ; Mirzarafie-Ahi, Ariana ; Parris, Elliott ; Prout, Luba ; Steijl, Barbara ; Wellman, Rachel ; Fan, Zhao ; Jing, Zhang ; Wei, Yang ; Yuanzhan, Yang ; Zhaosen, Wen ; Appletion, Evan ; Chen, Jeffrey ; Patil, Abha ; Priracha, Shaheer ; Ryan, Kate ; Salvador, Nick ; Viola, John ; Boralli, Camila Maria S ; Bramorski, Camila Barbosa ; Cancino-Bernardi, Juliana ; De Lima, Ana Laura ; Lins, Paula Maria Pincela ; Melo, Cristiane Casonato ; Mendonca, Deborah Cezar ; Mosqueiro, Thiago ; Silva, Everton ; Vasconcelos, Graziele ; Asthana, Ruchi ; Lee, Donna ; Yu, Michelle ; Choi, Peter ; Lau, Effie ; Lau, Kenneth ; Ying, Oscar ; Malone, Brandon ; Young, Paul ; Ceney, Aidan ; Hawthorne, Dakota ; Lian, Sharon ; Mellentine, Sam ; Miller, Dylan ; Moreira, Barbara Castro ; Peebles, Christie ; Smith, Olivia ; Walsh, Kevin ; Zimont, Allison ; Brasino, Michael ; Donovan, Michael ; Young, Hannah ; Bejvl, Jan ; Georgiev, Daniel ; Kasl, Hynek ; Pechotova, Katerina ; Pelisek, Vaclav ; Sosnova, Anna ; Zach, Pavel ; Ciesla, Anthony ; Hoover, Benjamin ; Chapman, Elliott ; Marles-Wright, Jon ; Moynihan, Vicky ; Owen, Liusaidh ; Rothschild-Mancinelli, Brooke ; Cuillery, Emilie ; Heng, Joseph ; Jacquot, Vincent ; Malsot, Paola ; Meli, Rocco ; Pulver, Cyril ; Sarfatis, Ari ; Steiner, Loic ; Steininger, Victor ; Van Tiel, Nina ; Thouvenin, Gregoire ; Uran, Axel ; Baumgartner, Lisa ; Fomitcheva, Anna ; Gerngross, Daniel ; Jagger, Verena ; Meier, Michael ; Michel, Anja ; Bird, Jasmine ; Brown, Bradley ; Burlington, Todd ; Herring, Daniel ; Slack, Joseph ; Westwood, Georgina ; Wojcik, Emilia ; Bender, Julian ; Donauer, Julia ; Emig, Ramona ; Jesser, Rabea ; Neumann, Julika ; Stuhn, Lara ; Hasegawa, Takema ; Kozakai, Tomoya ; Maruyama, Haruka ; Colloms, Sean ; Flynn, Charlotte ; Lomeikaite, Vilija ; Provan, James ; Ning, Kang ; Tang, Shuyan ; Wu, Guozhao ; Yang, Yunjun ; Zeng, Zhi ; Zhan, Yi ; Chu, Pan ; Li, Jun ; Yan, Keji ; Athale, Chaitanya A. ; Bodkhe, Swapnil ; Gangan, Manasi ; Gakhare, Harsh ; Jawale, Yash ; Kadam, Snehal ; Moghe, Prachiti ; Mundhe, Gayatri ; Khetan, Neha ; Phadke, Ira ; Uniyal, Prashant ; Zadey, Siddhesh ; Cottignie, Ines ; Deprez, Eline ; Deryckere, Astrid ; Janssens, Jasper ; Jonnaert, Frederik ; Malczewska, Katarzyna ; Pak, Thomas ; Robben, Johan ; Thirukkumaran, Ovia Margaret ; Van Deuren, Vincent ; Vandebroek, Laurens ; Van Hese, Laura ; Van Wonterghem, Laetitia ; Verschooten, Leen ; Wolter, Moritz ; Auty, Joss ; Badge, Richard ; Crawford, Liam ; Dalgleish, Raymond ; Evans, Amy ; Grundy, Cameron ; Kruczko, Charlie ; Karia, Payal ; Glaister, Graeme ; Hakstol, Rhys ; Mate, Seme ; Otero, Karin ; Smith, Dustin ; Tingley, Jeff ; Wieden, Hans Joachim ; Wang, Haotian ; Yao, Ningning ; Franz, Matthias ; Knoerlein, Anna ; Koutsoubelis, Nicolas ; Loechner, Anne C. ; Mundt, Max ; Richter, Alexandra ; Schauer, Oliver ; Buss, Marjorie ; Tangirala, Sivateja ; Teague, Brian ; Huang, Tianyi ; Song, Xinhao ; Wei, Yibing ; Zhang, Zhaoran ; Cao, Longzhi ; Li, Cheng ; Yang, Kang ; Chen, Zhiqin ; Fang, Yuxing ; Sun, Libo ; Wang, Weiyi ; Yang, Yang ; Adams, David ; Colls, Joshua ; Timmons, Joshua ; Urick, David ; Adrian, Julia Anna ; Akan, Madina ; Chahibi, Youssef ; Lale, Rahmi ; Le Doujet, Typhaine ; Roee, Marit Vaagen ; Abdirakhmanova, Altynay ; Orakov, Askarbek ; Zhailauova, Azhar ; Liang, Jinyang ; Ma, Yu ; Qin, Qikai ; Su, Yetian ; Han, Ju Yeon ; Hull, Raphaella ; Kong, Wei Chung ; Lu, Li Chieh ; Quinton, Duke ; Aubert, Pauline ; Bourdarias, Johan ; Bugaud, Olivier ; Demon-Chaine, Coralie ; Hatin, Isabelle ; Kaid-Slimane, Ibtissam ; Kang, Seong Koo ; Moatti, Audrey ; Ndiaye, Cheikh Fall ; Ananos, Mathilde ; Arkhipenko, Alexander ; Bailly, Valentin ; Caput, Jules ; Castillo, Javier ; Chapet-Batlle, Alma ; Cherrier, Floriane ; Demarta-Gatsi, Claudia ; Gopaul, Deshmukh ; Gugger, Muriel ; Lambert, Caroline ; Krauss, Lucas ; Vandendaele, Amelie ; Xiaojing, Li ; Xiaomei, Lin ; Xunxun, Luo ; Hansen, Anders Chr ; Kronborg, Tina ; Pettersen, Jens S. ; Calvet, Charles ; Devlin, Tyler Dae ; Fujishima, Kosuke ; Greenberg, Danny ; Ju, Tina ; Kent, Ryan ; Kunin, Daniel ; Lieberman, Erica ; McCutcheon, Griffin ; Nguyen, Thai ; Rothschild, Lynn ; Shih, Joseph D. ; Takahashi, Jack ; Thompson, Kirsten ; Tran, Forrest ; Xiang, Daniel ; Richter, Felix ; Xiaoran, Yang ; Xiangyue, Hu ; Deng, Changyuan ; Hua, Shuyu ; Li, Yumeng ; Meng, Xinyu ; Wang, Boxiang ; Wang, Yingqi ; Wang, Xuan ; Xu, Zixuan ; Yan, Jieyu ; Yan, Ming ; Zhou, Yineng ; Orozco, Edgar Alberto Alcalá ; Bermúdez, José Alberto Cristerna ; Gómez, Daniela Flores ; Castañeda, José Ernesto Hernández ; Navarro, Diana Clarisse Montaño ; Pérezávila, Juana Yessica ; Figueroa, María Fernanda Salazar ; Arroyo, María Fernanda Sánchez ; Montesinos, Oliva Angélica Sánchez ; Cruz, Ángel Farid Rojas ; Gutiérrez, Carlos Ramos ; Lona, Alonso Pérez ; Olivares, Fernanda Sotomayor ; Iniesta, Jorge Sebastián Rodríguez ; Silva, Juan Carlos Rueda ; Ayukawa, Shotaro ; Kashiwagi, Takahiro ; Kiga, Daisuke ; Minegishi, Misa ; Shinohara, Riku ; Tokuma, Hiraku ; Yamazaki, Yuta ; Yasunishi, Shuhei ; Zixuan, Erinn Sim ; Afzal, Remsha ; Carrigan, Matthew ; Moran, Barry ; Mucha, Marlena ; Petrauskas, Arnas ; Marsden, Stefan ; Post, Michelle ; Rodenburg, Anne ; Sanguesa, Hector ; Van Der Does, Marit ; Van Rijn, Erwin ; Van't Hof, Max ; De Bruin, Yeshi ; De Ferrante, Hans ; Elschot, Elles ; Jacobs, Laura ; Muller, Jan Willem ; Nooijens, Sjoerd ; Vaassen, Femke ; Van Der Putten, Cas ; Van Leeuwen, Esther ; Van Smeden, Laura ; Zhu, Kwan Kwan ; Sabath, Kevin ; Sporbeck, Katharina ; Von Kügelgen, Nicolai ; Wellinger, Lisa ; Braun, Stefanie ; Ho, Jack ; Mishra, Yash ; Sebastian, Mariola ; Von Chamier, Lucas ; Ahsan, Fasih M. ; Satyadi, Megan A. ; Gunadhi, Vivienne ; Kyriakakis, Phillip ; Lee, Jenny ; Thavarajah, Walter ; Abtahi, Kimia ; Hand, Robert ; Loke, Chun Mun ; Wahab, Adam ; Zhu, Iowis ; Del Bianco, Cristina ; Chizzolini, Fabio ; Godino, Elisa ; Lentini, Roberta ; Mansy, Sheref S. ; Martin, Noel Yeh ; Pegorar, Claudio Oss ; Cook, Alexander ; Kerns, Timothy ; Nielsen, Chad ; Paskett, Michael ; Torgesen, Alexander ; Lee, Stephen ; Ospovat, Ophir ; Raza, Sikandar ; Shaykevich, Daniel ; Shilts, Jarrod ; Bajorinaite, Barbora ; Bendorius, Mykolas ; Rauluseviciute, Ieva ; Savickyte, Ieva ; Tumas, Sarunas ; Buchser, William ; Cryan, Elli ; Golino, Caroline ; Halleran, Andrew ; Jacobs, Taylor ; LeFew, Michael ; Maniaci, Joe ; Marken, John ; Saha, Margaret ; Vij, Panya ; DeSanty, Kayla ; Mazza, Julie ; Jiang, Yue |
| Date: | 2016-03-01 |
| Language: | en |
| Pages: | 1-22 |
| Department: | BBN Technologies IGEM Foundation Synthace Ltd. Agilent Technologies Department of Biotechnology and Chemical Technology Helsinki Institute for Information Technology HIIT ATOMS Turkiye Boston University Carnegie Mellon University Technical University of Denmark Ludwig Maximilian University of Munich Middle East Technical University Sumbawa University of Technology Southern University of Science and Technology Sun Yat-Sen University Tecnológico de Monterrey Universidad Autonoma de Nuevo Leon University of Ottawa Universitat Politècnica de València Wageningen University and Research Centre Worcester Polytechnic Institute Xiamen University University of Texas at Austin Bielefeld University Birkbeck University of London USP-Brazil City University of Hong Kong Colorado State University CU Boulder Swiss Federal Institute of Technology Lausanne Swiss Federal Institute of Technology Zurich University of Exeter Indian Institute of Science Education and Research KU Leuven Massachusetts Institute of Technology Northeast Agricultural University Nanjing Agricultural University Norwegian University of Science and Technology Ocean University of China University of Southern Denmark Shenzhen Middle School - SZMS 15 Tokyo Institute of Technology Trinity College Dublin Delft University of Technology Eindhoven University of Technology Tuebingen University of California Los Angeles University of California San Diego University of Maryland University of Trento Vanderbilt University College of William and Mary Department of Bioproducts and Biosystems |
| Series: | PLOS ONE, Volume 11, issue 3 |
| ISSN: | 1932-6203 |
| DOI-number: | 10.1371/journal.pone.0150182 |
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Beal , J , Haddock-Angelli , T , Gershater , M , De Mora , K , Lizarazo , M , Hollenhorst , J , Rettberg , R , Demling , P , Hanke , R , Osthege , M , Schechtel , A , Sudarsan , S , Zimmermann , A , Gabryelczyk , B , Ikonen , M , Salmela , M , Acar , M , Aktas , M F , Bestepe , F , Ceylan , F S , Cigdem , S , Dohan , M , Elitok , M , Gunduz , M , Gunduz , E , Hatipoglu , O F , Kaya , T , Sayin , O , Tapan , S , Tereci , O F , Uçar , A , Yilmaz , M , Barrick , J , Gutierrez , A , Mishler , D , Monk , J , Mortensen , K , Shin , N , Watkins , E , Chen , Y , Jin , Y , Shi , Y , Zhang , H M , Ono , B , Paino , I M M , Ribovski , L , Silva , I , Zampronio , D K , Birkholz , N , Busche , R F , Konzock , O , Lippold , S , Ludwig , C , Philippi , M , Platz , L , Sigismund , C , Weber , S , Wehrs , M , Werchau , N , Wronska , A , Yen , Z Z , Agarwal , Y , Appleton , E , Densmore , D , Esmurria , A , Lewis , K , Pacheco , A , Bruchez , M , Peters , D , Telmer , C , Wang , L , Canas-Duarte , S , Giraldo-Perez , D , Gomez-Garzon , C , Madrid-Wolff , J , Marin-Medina , N , Mazzanti , V , Rodriguez-Forero , L , Scher , E , Dowell , R , O'Hara , S , Pogoda , C S , Shattuck , K , Altintas , A , Bali , A P , Bech , R , Egholm , A , Hansen , A S L , Jensen , K , Karlsen , K B , Mosbech , C , Belkhelfa , S , Berenger , N , Bodinier , R , Jacry , C , Matabishi-Bibi , L , Parutto , P , Zaworski , J , De Vries , A , De Wijs , F , Elbert , R , Hielkema , L , Jagadeesan , C , Jayawardhana , B , Kuipers , O , Lauxen , A , Meijer , T , Mous , S , Van Raaphorst , R , Saraf , A , Schepers , O , Smits , O , Veening , J W , Weme , R D O , Wieske , L , Ainsworth , C , Spencer-Milnes , X , Ávila , A G , Gamez , E C , Huerta , A L T , Ramirez , C A M , Popp , P , Radeck , J , Sommer , A , Li , X , Wu , Q , Zhao , H , Zhao , R , Bastuzel , I , Ceyhan , Y , Gursel , M , Kizil , B , Kumru , I , Kuvvet , Y , Tercan , H , Yuksel , S , Niyazmetova , L , Ang , T , Black , L , Kelly , C , Wadhams , G , Basier , C , Czerwinska , U , Ananda , C S , Al Azhar , M , Elviantari , A , Fitriana , M , Witarto , A B , Yulianti , Hou , Q , Pei , W , Rifei , C , Rong , W , Wei , H , Yushan , Z , Jianguo , H , Lai , D , Li , P , Liu , J , Ni , C , Zhang , Q , Cadenas , C , Canabal , E J Z , Cantu , C N A , Cantu , M A V , Canedo , E C , Cordova , C M V , De La Paz Espinosa , J A , Garcia , C E A , Heredia , A L N , Hernandez , A , Jauregui , S V , Montiel , E R , Morales , E S , Morales , Y M C , Pena , O A C , Ramirez-Rodríguez , E A , Trejo , E V , Ceja , J G R , Hernandez , J E M , Hernandez , M A P , Perez , E A , Ramirez , R P T , Rocha , J C M , Sanchez , A , Vazquez , C M G , Hanzel , M , Mohand-Said , S , Sawar , S , Siriwardena , D , Tzahristos , A , Anlind , N , Friberg , M , Gullberg , E , Herman , S , Christensen , D , Gertsch , S , Maxfield , C , Miller , C , Putman , R , Bauerl , C , Lopez , L T E , Huet-Trujillo , E , Vilar , M V , Birk , M S , Claassens , N , De Koster , W , Van Rosmalen , R , Wu , W Y , Davies , S , Goss , D , Rostain , W , Tye , C , Yousaf , W , Farny , N , LaJeunesse , C , Turland , A , Chen , A , Chen , J , Chen , Y , Chen , Z , Fang , B , Fu , X , Guo , X , Lei , Y , Li , J , Li , Z , Liu , C , Liu , W , Liu , Y , Lv , Y , Ruan , Q , Su , Y , Tang , C , Wang , Y , Wu , F , Yan , X , Zhang , R , Zhang , T , Isaacs , F , Leyva-Hernandez , A , Ma , N , Mao , S , Naidu , Y , Miinalainen , T , Aruanno , M , Calendini , D , Chabert , Y , Chambonnier , G , Choukour , M , De Gaulejac , E , Houy , C , Levier , A , Logger , L , Nin , S , Prima , V , Sturgis , J N , Fang , B , Cigdem , S , Gutierrez , A , Poondla , R , Reddy , S , Rocha , T , Schulte , N , Wehle , D , Jackowski , M E , Craig , S R , Mirzarafie-Ahi , A , Parris , E , Prout , L , Steijl , B , Wellman , R , Fan , Z , Jing , Z , Wei , Y , Yuanzhan , Y , Zhaosen , W , Appletion , E , Chen , J , Patil , A , Priracha , S , Ryan , K , Salvador , N , Viola , J , Boralli , C M S , Bramorski , C B , Cancino-Bernardi , J , De Lima , A L , Lins , P M P , Melo , C C , Mendonca , D C , Mosqueiro , T , Silva , E , Vasconcelos , G , Asthana , R , Lee , D , Yu , M , Choi , P , Lau , E , Lau , K , Ying , O , Malone , B , Young , P , Ceney , A , Hawthorne , D , Lian , S , Mellentine , S , Miller , D , Moreira , B C , Peebles , C , Smith , O , Walsh , K , Zimont , A , Brasino , M , Donovan , M , Young , H , Bejvl , J , Georgiev , D , Kasl , H , Pechotova , K , Pelisek , V , Sosnova , A , Zach , P , Ciesla , A , Hoover , B , Chapman , E , Marles-Wright , J , Moynihan , V , Owen , L , Rothschild-Mancinelli , B , Cuillery , E , Heng , J , Jacquot , V , Malsot , P , Meli , R , Pulver , C , Sarfatis , A , Steiner , L , Steininger , V , Van Tiel , N , Thouvenin , G , Uran , A , Baumgartner , L , Fomitcheva , A , Gerngross , D , Jagger , V , Meier , M , Michel , A , Bird , J , Brown , B , Burlington , T , Herring , D , Slack , J , Westwood , G , Wojcik , E , Bender , J , Donauer , J , Emig , R , Jesser , R , Neumann , J , Stuhn , L , Hasegawa , T , Kozakai , T , Maruyama , H , Colloms , S , Flynn , C , Lomeikaite, V , Provan , J , Ning , K , Tang , S , Wu , G , Yang , Y , Zeng , Z , Zhan , Y , Chu , P , Li , J , Yan , K , Athale , C A , Bodkhe , S , Gangan , M , Gakhare , H , Jawale , Y , Kadam , S , Moghe , P , Mundhe , G , Khetan , N , Phadke , I , Uniyal , P , Zadey , S , Cottignie , I , Deprez , E , Deryckere , A , Janssens , J , Jonnaert , F , Malczewska , K , Pak , T , Robben , J , Thirukkumaran , O M , Van Deuren , V , Vandebroek , L , Van Hese , L , Van Wonterghem , L , Verschooten , L , Wolter , M , Auty , J , Badge , R , Crawford , L , Dalgleish , R , Evans , A , Grundy , C , Kruczko , C , Karia , P , Glaister , G , Hakstol , R , Mate , S , Otero , K , Smith , D , Tingley , J , Wieden , H J , Wang , H , Yao , N , Franz , M , Knoerlein , A , Koutsoubelis , N , Loechner , A C , Mundt , M , Richter , A , Schauer , O , Buss , M , Tangirala , S , Teague , B , Huang , T , Song , X , Wei , Y , Zhang , Z , Cao , L , Li , C , Yang , K , Chen , Z , Fang , Y , Sun , L , Wang , W , Yang , Y , Adams , D , Colls , J , Timmons , J , Urick , D , Adrian , J A , Akan , M , Chahibi , Y , Lale , R , Le Doujet , T , Roee , M V , Abdirakhmanova , A , Orakov , A , Zhailauova , A , Liang , J , Ma , Y , Qin , Q , Su , Y , Han , J Y , Hull , R , Kong , W C , Lu , L C , Quinton , D , Aubert , P , Bourdarias , J , Bugaud , O , Demon-Chaine , C , Hatin , I , Kaid-Slimane , I , Kang , S K , Moatti , A , Ndiaye , C F , Ananos , M , Arkhipenko , A , Bailly , V , Caput , J , Castillo , J , Chapet-Batlle , A , Cherrier , F , Demarta-Gatsi , C , Gopaul , D , Gugger , M , Lambert , C , Krauss , L , Vandendaele , A , Xiaojing , L , Xiaomei , L , Xunxun , L , Hansen , A C , Kronborg , T , Pettersen , J S , Calvet , C , Devlin , T D , Fujishima , K , Greenberg , D , Ju , T , Kent , R , Kunin , D , Lieberman , E , McCutcheon , G , Nguyen , T , Rothschild , L , Shih , J D , Takahashi , J , Thompson , K , Tran , F , Xiang , D , Richter , F , Xiaoran , Y , Xiangyue , H , Deng , C , Hua , S , Li , Y , Meng , X , Wang , B , Wang , Y , Wang , X , Xu , Z ,Yan , J , Yan , M , Zhou , Y , Orozco , E A A , Bermúdez , J A C , Gómez , D F , Castañeda , J E H , Navarro , D C M , Pérezávila , J Y , Figueroa , M F S , Arroyo , M F S , Montesinos , O A S , Cruz , Á F R , Gutiérrez , C R , Lona , A P , Olivares , F S , Iniesta , J S R , Silva , J C R , Ayukawa , S , Kashiwagi , T , Kiga , D , Minegishi , M , Shinohara , R , Tokuma , H , Yamazaki , Y , Yasunishi , S , Zixuan , E S , Afzal , R , Carrigan , M , Moran , B , Mucha , M , Petrauskas , A , Marsden , S , Post , M , Rodenburg , A , Sanguesa , H , Van Der Does , M , Van Rijn , E , Van't Hof , M , De Bruin , Y , De Ferrante , H , Elschot , E , Jacobs , L , Muller , J W , Nooijens , S , Vaassen , F , Van Der Putten , C , Van Leeuwen , E , Van Smeden , L , Zhu , K K , Sabath , K , Sporbeck , K , Von Kügelgen , N , Wellinger , L , Braun , S , Ho , J , Mishra , Y , Sebastian , M , Von Chamier , L , Ahsan , F M , Satyadi , M A , Gunadhi , V , Kyriakakis , P , Lee , J , Thavarajah , W , Abtahi , K , Hand , R , Loke , C M, Wahab , A , Zhu , I , Del Bianco , C , Chizzolini , F , Godino , E , Lentini , R , Mansy , S S , Martin , N Y , Pegorar , C O , Cook , A , Kerns , T , Nielsen , C , Paskett , M , Torgesen , A , Lee , S , Ospovat , O , Raza , S , Shaykevich , D , Shilts , J , Bajorinaite , B , Bendorius , M , Rauluseviciute , I , Savickyte , I , Tumas , S , Buchser , W , Cryan , E , Golino , C , Halleran , A , Jacobs , T , LeFew , M , Maniaci , J , Marken , J , Saha , M , Vij , P , DeSanty , K , Mazza , J , Jiang , Y , Liu , Y & Yang , Y 2016 , ' Reproducibility of fluorescent expression from engineered biological constructs in E. coli ' , PloS one , vol. 11 , no. 3 , e0150182 , pp. 1-22 . https://doi.org/10.1371/journal.pone.0150182 |
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Abstract:We present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions around the world measured fluorescence from three engineered constitutive constructs in E. coli. Few participants were able to measure absolute fluorescence, so data was analyzed in terms of ratios. Precision was strongly related to fluorescent strength, ranging from 1.54-fold standard deviation for the ratio between strong promoters to 5.75-fold for the ratio between the strongest and weakest promoter, and while host strain did not affect expression ratios, choice of instrument did. This result shows that high quantitative precision and reproducibility of results is possible, while at the same time indicating areas needing improved laboratory practices.
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