Biomimetic collagen I and IV double layer Langmuir-Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells

 |  Login

Show simple item record

dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Sorkio, Anni E.
dc.contributor.author Vuorimaa-Laukkanen, Elina P.
dc.contributor.author Hakola, Hanna M.
dc.contributor.author Liang, Huamin
dc.contributor.author Ujula, Tiina A.
dc.contributor.author Valle-Delgado, Juan Jose
dc.contributor.author Osterberg, Monika
dc.contributor.author Yliperttula, Marjo L.
dc.contributor.author Skottman, Heli
dc.date.accessioned 2017-05-11T09:09:42Z
dc.date.available 2017-05-11T09:09:42Z
dc.date.issued 2015-05
dc.identifier.citation Sorkio , A E , Vuorimaa-Laukkanen , E P , Hakola , H M , Liang , H , Ujula , T A , Valle-Delgado , J J , Osterberg , M , Yliperttula , M L & Skottman , H 2015 , ' Biomimetic collagen I and IV double layer Langmuir-Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells ' BIOMATERIALS , vol 51 , pp. 257-269 . DOI: 10.1016/j.biomaterials.2015.02.005 en
dc.identifier.issn 0142-9612
dc.identifier.issn 1878-5905
dc.identifier.other PURE UUID: dcfe8821-2e0b-416a-84f2-e86573da9e01
dc.identifier.other PURE ITEMURL: https://research.aalto.fi/en/publications/biomimetic-collagen-i-and-iv-double-layer-langmuirschaefer-films-as-microenvironment-for-human-pluripotent-stem-cell-derived-retinal-pigment-epithelial-cells(dcfe8821-2e0b-416a-84f2-e86573da9e01).html
dc.identifier.other PURE FILEURL: https://research.aalto.fi/files/6736029/Accepted_paper.pdf
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/25905
dc.description.abstract The environmental cues received by the cells from synthetic substrates in vitro are very different from those they receive in vivo. In this study, we applied the LangmuireSchaefer (LS) deposition, a variant of LangmuireBlodgett technique, to fabricate a biomimetic microenvironment mimicking the structure and organization of native Bruch's membrane for the production of the functional human embryonic stem cell derived retinal pigment epithelial (hESC-RPE) cells. Surface pressure-area isotherms were measured simultaneously with Brewster angle microscopy to investigate the self-assembly of human collagens type I and IV on air-subphase interface. Furthermore, the structure of the prepared collagen LS films was characterized with scanning electron microscopy, atomic force microscopy, surface plasmon resonance measurements and immunofluorescent staining. The integrity of hESC-RPE on double layer LS films was investigated by measuring transepithelial resistance and permeability of small molecular weight substance. Maturation and functionality of hESC-RPE cells on double layer collagen LS films was further assessed by RPE-specific gene and protein expression, growth factor secretion, and phagocytic activity. Here, we demonstrated that the prepared collagen LS films have layered structure with oriented fibers corresponding to architecture of the uppermost layers of Bruch's membrane and result in increased barrier properties and functionality of hESC-RPE cells as compared to the commonly used dip-coated controls. en
dc.format.extent 13
dc.format.extent 257-269
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries BIOMATERIALS en
dc.relation.ispartofseries Volume 51 en
dc.rights openAccess en
dc.subject.other 216 Materials engineering en
dc.subject.other 215 Chemical engineering en
dc.title Biomimetic collagen I and IV double layer Langmuir-Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Department of Forest Products Technology
dc.contributor.department Department of Bioproducts and Biosystems en
dc.subject.keyword Biomimetic material
dc.subject.keyword Collagen structure
dc.subject.keyword Langmuir Blodgett film
dc.subject.keyword Retinal pigment epithelial cell
dc.subject.keyword Retina
dc.subject.keyword Human embryonic stem cell
dc.subject.keyword 216 Materials engineering
dc.subject.keyword 215 Chemical engineering
dc.identifier.urn URN:NBN:fi:aalto-201705114280
dc.identifier.doi 10.1016/j.biomaterials.2015.02.005
dc.date.embargo info:eu-repo/date/embargoEnd/2017-05-31


Files in this item

Files Size Format View

There are no open access files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search archive


Advanced Search

article-iconSubmit a publication

Browse