Computational fluid dynamic modelling of a pilot-scale bioreactor

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.advisor Spann, Robert
dc.contributor.advisor Yang, Jifeng
dc.contributor.author Han, Pinxiang
dc.date.accessioned 2016-09-22T08:52:41Z
dc.date.available 2016-09-22T08:52:41Z
dc.date.issued 2016-08-23
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/22137
dc.description.abstract The master's thesis focuses on the investigation of mixing time of a fermentation process using lactic acid bacteria (LAB). Due to the microorganism growth, acid is produced in the process and base is added frequently into the bioreactor for pH control. In this case, gradients of substrate, product and pH might exist in the system. The gradients can result in negative effect on the microorganism growth and process productivity. Thus, it is important to investigate the mixing performance of the bioreactor. Computational fluid dynamic (CFD) modelling is applied in this thesis to predict fluid flow behavior and mixing time of the pilot-scale bioreactor. The lactic acid bacteria (LAB) fermentation is anaerobic and a one phase CFD model is built based on the pilot-scale bioreactor geometry. The model mesh elements, mesh quality and type and ANSYS CFX settings were studied to get an accurate and reliable CFD model. Pilot-scale mixing experiments were carried out in order to validate the CFD model. Six pH sensors were mounted on a rack measuring pH at different locations in the bioreactor. Base was added from the top and bottom of the bioreactor at 200rpm and 100rpm impeller speed. pH dynamic data with 1s sampling interval were collected. CFD simulations were performed at the same conditions as in the pilot experiments. The concentration of tracer at each time step and the calculated mixing time were compared with the pilot experiments for validation. In the final part of the project, the CFD model is combined a with LAB kinetic model to predict the kinetics of LAB growth and substrate consumption in the bioreactor. The combined CFD and kinetic model was compared at 200rpm, 100rpm and without the fluid flow fields. en
dc.format.extent 74+15
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.title Computational fluid dynamic modelling of a pilot-scale bioreactor en
dc.type G2 Pro gradu, diplomityö fi
dc.contributor.school Kemian tekniikan korkeakoulu fi
dc.subject.keyword computational fluid dynamics en
dc.subject.keyword lactic acid bacteria fermentation en
dc.subject.keyword mixing time en
dc.subject.keyword pH gradient en
dc.subject.keyword pilot-scale bioreactor en
dc.identifier.urn URN:NBN:fi:aalto-201609224145
dc.programme.major Process Systems Engineering fi
dc.programme.mcode KE3004 fi
dc.type.ontasot Master's thesis en
dc.type.ontasot Diplomityö fi
dc.contributor.supervisor Alopaeus, Ville
dc.programme Master's Programme in Chemical Technology fi
dc.location PK fi


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