High-resolution analysis of the human intestinal microbiota stability landscape

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.advisor Lahti, Leo
dc.contributor.author Blake, Tineka
dc.date.accessioned 2015-09-18T09:13:32Z
dc.date.available 2015-09-18T09:13:32Z
dc.date.issued 2015-08-27
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/17836
dc.description.abstract The intestinal microbiota is a complex microbial ecosystem that occupies the human host and is essential for health and well-being. More specifically, it plays a dominant role in nutrition, development, metabolism, pathogen resistance and the regulation of immune response. Recent technological advances have aided our discovery of these vastly diverse microbial communities and their importance to host physiology has been unveiled. However, the mechanisms that control this complex ecosystem are yet to be fully elucidated. Experimental evidence supports the presence of bistable abundance distributions in the intestinal microbiota. Some bacterial taxa exist in either a high or low abundance state with decreased stability observed at the intermediate abundance range. Since bistability is relatively difficult to measure in the human gut microbiota (due to the lack of longitudinal data made readily available), bistability can be inferred from observations of bimodality in vastly accessible static data. In addition to this, the quantification of the unstable region observed at the intermediate abundance range and the assessment of state stability and transitions provides us with further evidence to support the concept alternative stable states within the human gut. Here, bimodality detection, intermediate stability and multi-state modelling have been implemented upon high-resolution microarray and next generation sequencing microbiota profiling data in attempt to identify bistable groups in sub-communities of intestinal microbes. The aim of this thesis is to identify novel bistable taxa along with the validation of pre-existing bistable groups. It is also assess how bistability is best observed in this complex nonlinear dynamical system. The bistable taxa appearing here have provided us with candidates for the manipulation of the intestinal microbiota for desired health outcomes. Furthermore, their robustness also serves as an indicator of the overall ecosystem state providing great diagnostic potential. en
dc.format.extent 52+2
dc.language.iso en en
dc.title High-resolution analysis of the human intestinal microbiota stability landscape en
dc.type G2 Pro gradu, diplomityö en
dc.contributor.school Perustieteiden korkeakoulu fi
dc.subject.keyword human gut microbiota en
dc.subject.keyword bimodality en
dc.subject.keyword bistability en
dc.subject.keyword temporal dynamics en
dc.identifier.urn URN:NBN:fi:aalto-201509184439
dc.programme.major Computational and Systems Biology fi
dc.programme.mcode IL3013 fi
dc.type.ontasot Master's thesis en
dc.type.ontasot Diplomityö fi
dc.contributor.supervisor Rousu, Juho
dc.programme Master's Degree Programme in Computational and Systems Biology (euSYSBIO) fi

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