Abstract:
Relying on previous publications and tools (Bob, 2010; Costes et al., 2004; Dunn et al., 2011; Fiji/ImageJ program web site, 2020; Herbert, undated; Fiji/ImageJ program documentation, 2020; Online Manual for the WCIF-ImageJ collection, undated; Stauffer et al. 2018), this current document describes a method developed by Lauri Lahti and how he used this method to perform the co-localization analysis and create the results that are reported in the research article Abouelezz, Stefen, Segerstråle, Micinski, Minkeviciene, Lahti, Hardeman, Gunning, Hoogenraad, Taira, Fath, & Hotulainen (2020), "Tropomyosin Tpm3.1 Is Required to Maintain the Structure and Function of the Axon Initial Segment".