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Graphene Biosensor Programming with Genetically Engineered Fusion Protein Monolayers

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Soikkeli, Miika
dc.contributor.author Kurppa, Katri
dc.contributor.author Kainlauri, Markku
dc.contributor.author Arpiainen, Sanna
dc.contributor.author Paananen, Arja
dc.contributor.author Gunnarsson, David
dc.contributor.author Joensuu, Jussi J.
dc.contributor.author Laaksonen, Päivi
dc.contributor.author Prunnila, Mika
dc.contributor.author Linder, Markus B.
dc.contributor.author Ahopelto, Jouni
dc.date.accessioned 2021-04-20T06:49:30Z
dc.date.available 2021-04-20T06:49:30Z
dc.date.issued 2016-03-30
dc.identifier.citation Soikkeli , M , Kurppa , K , Kainlauri , M , Arpiainen , S , Paananen , A , Gunnarsson , D , Joensuu , J J , Laaksonen , P , Prunnila , M , Linder , M B & Ahopelto , J 2016 , ' Graphene Biosensor Programming with Genetically Engineered Fusion Protein Monolayers ' , ACS Applied Materials and Interfaces , vol. 8 , no. 12 , pp. 8257-8264 . https://doi.org/10.1021/acsami.6b00123 en
dc.identifier.issn 1944-8244
dc.identifier.issn 1944-8252
dc.identifier.other PURE UUID: b16cbb38-d27a-40bb-acee-f59cb7aad932
dc.identifier.other PURE ITEMURL: https://research.aalto.fi/en/publications/b16cbb38-d27a-40bb-acee-f59cb7aad932
dc.identifier.other PURE LINK: http://www.scopus.com/inward/record.url?scp=84963783524&partnerID=8YFLogxK
dc.identifier.other PURE FILEURL: https://research.aalto.fi/files/61703730/acsami.6b00123_2.pdf
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/106913
dc.description.abstract We demonstrate a label-free biosensor concept based on specific receptor modules, which provide immobilization and selectivity to the desired analyte molecules, and on charge sensing with a graphene field effect transistor. The receptor modules are fusion proteins in which small hydrophobin proteins act as the anchor to immobilize the receptor moiety. The functionalization of the graphene sensor is a single-step process based on directed self-assembly of the receptor modules on a hydrophobic surface. The modules are produced separately in fungi or plants and purified before use. The modules form a dense and well-oriented monolayer on the graphene transistor channel and the receptor module monolayer can be removed, and a new module monolayer with a different selectivity can be assembled in situ. The receptor module monolayers survive drying, showing that the functionalized devices can be stored and have a reasonable shelf life. The sensor is tested with small charged peptides and large immunoglobulin molecules. The measured sensitivities are in the femtomolar range, and the response is relatively fast, of the order of one second. (Graph Presented). en
dc.format.extent 8
dc.format.extent 8257-8264
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries ACS Applied Materials and Interfaces en
dc.relation.ispartofseries Volume 8, issue 12 en
dc.rights openAccess en
dc.title Graphene Biosensor Programming with Genetically Engineered Fusion Protein Monolayers en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department VTT Technical Research Centre of Finland
dc.contributor.department Nanostructures and Materials
dc.contributor.department Department of Biotechnology and Chemical Technology
dc.contributor.department Department of Bioproducts and Biosystems en
dc.subject.keyword graphene
dc.subject.keyword biosensor
dc.subject.keyword fusion protein
dc.subject.keyword hydrophobin
dc.subject.keyword self-assembly
dc.subject.keyword Debye length
dc.subject.keyword CRYSTAL-STRUCTURES
dc.subject.keyword HYDROPHOBIN HFBI
dc.subject.keyword SENSORS
dc.subject.keyword FUNCTIONALIZATION
dc.subject.keyword PURIFICATION
dc.subject.keyword FILMS
dc.identifier.urn URN:NBN:fi:aalto-202104206207
dc.identifier.doi 10.1021/acsami.6b00123
dc.type.version publishedVersion

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