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Substrate specificities of inteins investigated by QuickDrop-cassette mutagenesis

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Oeemig, Jesper S.
dc.contributor.author Beyer, Hannes M.
dc.contributor.author Aranko, A. Sesilja
dc.contributor.author Mutanen, Justus
dc.contributor.author Iwaï, Hideo
dc.date.accessioned 2021-01-25T10:11:15Z
dc.date.available 2021-01-25T10:11:15Z
dc.date.issued 2020-10-01
dc.identifier.citation Oeemig , J S , Beyer , H M , Aranko , A S , Mutanen , J & Iwaï , H 2020 , ' Substrate specificities of inteins investigated by QuickDrop-cassette mutagenesis ' , FEBS Letters , vol. 594 , no. 20 , pp. 3338-3355 . https://doi.org/10.1002/1873-3468.13909 en
dc.identifier.issn 0014-5793
dc.identifier.other PURE UUID: 49033568-6663-4914-be19-9e4a0e941a17
dc.identifier.other PURE ITEMURL: https://research.aalto.fi/en/publications/49033568-6663-4914-be19-9e4a0e941a17
dc.identifier.other PURE LINK: http://www.scopus.com/inward/record.url?scp=85091468954&partnerID=8YFLogxK
dc.identifier.other PURE FILEURL: https://research.aalto.fi/files/55034794/1873_3468.13909.pdf
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/102139
dc.description.abstract Inteins catalyze self-excision from host precursor proteins while concomitantly ligating the flanking substrates (exteins) with a peptide bond. Noncatalytic extein residues near the splice junctions, such as the residues at the −1 and +2 positions, often strongly influence the protein-splicing efficiency. The substrate specificities of inteins have not been studied for many inteins. We developed a convenient mutagenesis platform termed “QuickDrop”-cassette mutagenesis for investigating the influences of 20 amino acid types at the −1 and +2 positions of different inteins. We elucidated 17 different profiles of the 20 amino acid dependencies across different inteins. The substrate specificities will accelerate our understanding of the structure–function relationship at the splicing junctions for broader applications of inteins in biotechnology and molecular biosciences. en
dc.format.extent 18
dc.format.extent 3338-3355
dc.format.mimetype application/pdf
dc.language.iso en en
dc.publisher Elsevier Science B.V.
dc.relation.ispartofseries FEBS Letters en
dc.relation.ispartofseries Volume 594, issue 20 en
dc.rights openAccess en
dc.title Substrate specificities of inteins investigated by QuickDrop-cassette mutagenesis en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department University of Helsinki
dc.contributor.department Department of Bioproducts and Biosystems
dc.subject.keyword intein
dc.subject.keyword mutagenesis
dc.subject.keyword protein ligation
dc.subject.keyword protein splicing
dc.subject.keyword substrate specificity
dc.identifier.urn URN:NBN:fi:aalto-202101251449
dc.identifier.doi 10.1002/1873-3468.13909
dc.type.version publishedVersion

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