Browsing by Author "Zhang, Hongbo"

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  • Cellular delivery of enzyme-loaded DNA origami
    (2016) Ora, Ari; Järvihaavisto, Erika; Zhang, Hongbo; Auvinen, Henni; Santos, Hélder A.; Kostiainen, Mauri A.; Linko, Veikko
     School of Chemical Technology | A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
  • Manipulating Superparamagnetic Microparticles with an Electromagnetic Needle
    (2017-10-16) Cenev, Zoran; Zhang, Hongbo; Sariola, Veikko; Rahikkala, Antti; Liu, Dongfei; Santos, Hélder A.; Zhou, Quan
     A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
    Selective, precise, and high-throughput manipulation of individual superparamagnetic microparticles has profound applications in performing location-tailored in vitro biomedical studies. The current techniques for manipulation of microparticles allow only a single particle in the manipulation workspace, or simultaneous transportation of multiple microparticles in batches. In this work, a method based on a robotized electromagnetic needle for manipulation of individual superparamagnetic microparticles within a microparticle population is introduced. By automatically controlling the highly localized magnetic field of the needle, a single microparticle is selectively picked when its neighboring particle is few micrometers away. Supported by the nanometer resolution of the robotic positioner, particles are placed at sub-micrometer precision. This manipulation technique allows the creating of arbitrary patterns, sorting of microparticles based on size and morphology, and transporting of individual microparticles in 3D space. Therefore, this approach has the potential to enable more deterministic and quantitative microanalysis and microsynthesis using superparamagnetic microparticles.
  • Protein Coating of DNA Nanostructures for Enhanced Stability and Immunocompatibility
    (2017-09) Auvinen, Henni; Zhang, Hongbo; Nonappa, Nonappa; Kopilow, Alisa; Niemelä, Elina H.; Nummelin, Sami; Correia, Alexandra Mr; Santos, Hélder A.; Linko, Veikko; Kostiainen, Mauri A.
     A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
    Fully addressable DNA nanostructures, especially DNA origami, possess huge potential to serve as inherently biocompatible and versatile molecular platforms. However, their use as delivery vehicles in therapeutics is compromised by their low stability and poor transfection rates. This study shows that DNA origami can be coated by precisely defined one-to-one protein-dendron conjugates to tackle the aforementioned issues. The dendron part of the conjugate serves as a cationic binding domain that attaches to the negatively charged DNA origami surface via electrostatic interactions. The protein is attached to dendron through cysteine-maleimide bond, making the modular approach highly versatile. This work demonstrates the coating using two different proteins: bovine serum albumin (BSA) and class II hydrophobin (HFBI). The results reveal that BSA-coating significantly improves the origami stability against endonucleases (DNase I) and enhances the transfection into human embryonic kidney (HEK293) cells. Importantly,it is observed that BSA-coating attenuates the activation of immune response in mouse primary splenocytes. Serum albumin is the most abundant protein in the blood with a long circulation half-life and has already found clinically approved applications in drug delivery. It is therefore envisioned that the proposed system can open up further opportunities to tune the properties of DNA nanostructures in biological environment, and enable their use in various delivery applications.
  • Protein Coating of DNA Nanostructures for Enhanced Stability and Immunocompatibility
    (2017) Auvinen, Henni; Zhang, Hongbo; Nonappa, Nonappa; Kopilow, Alisa; Niemelä, Elina H.; Nummelin, Sami; Correia, Alexandra; Santos, Hélder A.; Linko, Veikko; Kostiainen, Mauri A.
     School of Science | A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä