Browsing by Author "Luotonen, Otso I.V."

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  • Benchmarking supramolecular adhesive behavior of nanocelluloses, cellulose derivatives and proteins
    (2022-09-15) Luotonen, Otso I.V.; Greca, Luiz G.; Nyström, Gustav; Guo, Junling; Richardson, Joseph J.; Rojas, Orlando J.; Tardy, Blaise L.
     A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
    One of the key steps towards a broader implementation of renewable materials is the development of biodegradable adhesives that can be attained at scale and utilized safely. Recently, cellulose nanocrystals (CNCs) were demonstrated to have remarkable adhesive properties. Herein, we study three classes of naturally synthesized biopolymers as adhesives, namely nanocelluloses (CNFs), cellulose derivatives, and proteins by themselves and when used as additives with CNCs. Among the samples evaluated, the adhesion strength was the highest for bovine serum albumin and hydroxypropyl cellulose (beyond 10 MPa). These were followed by carboxymethylcellulose and CNCs (ca. 5 MPa) and mechanically fibrillated CNFs (ca. 2 MPa), and finally by tempo-oxidized CNFs (0.2 MPa) and lysozyme (1.5 MPa). Remarkably, we find that the anisotropy of adhesion (in plane vs out of plane) falls within a narrow range across the bio-based adhesives studied. Collectively, this study benchmarks bio-based non-covalent adhesives aiming towards their improvement and implementation.
  • Resolving host-guest interactions between pillararenes and homoserine lactones to restrain bacterial quorum sensing
    (2024-07-17) Luotonen, Otso I.V.; Osmekhina, Ekaterina; Anaya-Plaza, Eduardo; Kaabel, Sandra; Harmat, Adam L.; Sammalkorpi, Maria; Jonkergouw, Christopher; Linder, Markus B.; Kostiainen, Mauri A.
     A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
    Using supramolecular host molecules to hinder bacterial quorum sensing (QS) is a potential approach in circumventing antimicrobial resistance (AMR). The emergent family of pillararenes offers promising candidates for binding homoserine lactones (HSLs), signaling molecules used by gram-negative species, including WHO critical-priority antibiotic-resistant bacteria. Here, we compare seven cationic pillararenes against four HSLs, from (supra)molecular interactions to biological assays. Complexation, characterized by dye displacement assay and NMR spectroscopy, complemented by all-atom molecular dynamics (MD) simulations, was compared to effects in biological systems, studied using a bacterial HSL reporter system as well as biofilm and pyocyanin assays as models of QS-mediated virulence. HSL binding improves approximately 10-fold versus previous reports with a hydroxyl-functionalized pillararene, and a deeper-cavity host with marked preference for the longest-tailed HSL is identified. Successful HSL capture is directly reflected as impaired biofilm formation and pyocyanin production and improved healing in open wound in vivo models.