Browsing by Author "Bhosale, Santosh D."

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Quantitative proteomic characterization and comparison of T helper 17 and induced regulatory T cells
(2018-05-31) Mohammad, Imran; Nousiainen, Kari; Bhosale, Santosh D.; Starskaia, Inna; Moulder, Robert; Rokka, Anne; Cheng, Fang; Mohanasundaram, Ponnuswamy; Eriksson, John E.; Goodlett, David R.; Lähdesmäki, Harri; Chen, Zhi
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
The transcriptional network and protein regulators that govern T helper 17 (Th17) cell differentiation have been studied extensively using advanced genomic approaches. For a better understanding of these biological processes, we have moved a step forward, from gene- to protein-level characterization of Th17 cells. Mass spectrometry–based label-free quantitative (LFQ) proteomics analysis were made of in vitro differentiated murine Th17 and induced regulatory T (iTreg) cells. More than 4,000 proteins, covering almost all subcellular compartments, were detected. Quantitative comparison of the protein expression profiles resulted in the identification of proteins specifically expressed in the Th17 and iTreg cells. Importantly, our combined analysis of proteome and gene expression data revealed protein expression changes that were not associated with changes at the transcriptional level. Our dataset provides a valuable resource, with new insights into the proteomic characteristics of Th17 and iTreg cells, which may prove useful in developing treatment of autoimmune diseases and developing tumor immunotherapy.
Serum APOC1 levels are decreased in young autoantibody positive children who rapidly progress to type 1 diabetes
(2023-12) Hirvonen, M. Karoliina; Lietzén, Niina; Moulder, Robert; Bhosale, Santosh D.; Koskenniemi, Jaakko; Vähä-Mäkilä, Mari; Nurmio, Mirja; Orešič, Matej; Ilonen, Jorma; Toppari, Jorma; Veijola, Riitta; Hyöty, Heikki; Lähdesmäki, Harri; Knip, Mikael; Cheng, Lu; Lahesmaa, Riitta
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Better understanding of the early events in the development of type 1 diabetes is needed to improve prediction and monitoring of the disease progression during the substantially heterogeneous presymptomatic period of the beta cell damaging process. To address this concern, we used mass spectrometry-based proteomics to analyse longitudinal pre-onset plasma sample series from children positive for multiple islet autoantibodies who had rapidly progressed to type 1 diabetes before 4 years of age (n = 10) and compared these with similar measurements from matched children who were either positive for a single autoantibody (n = 10) or autoantibody negative (n = 10). Following statistical analysis of the longitudinal data, targeted serum proteomics was used to verify 11 proteins putatively associated with the disease development in a similar yet independent and larger cohort of children who progressed to the disease within 5 years of age (n = 31) and matched autoantibody negative children (n = 31). These data reiterated extensive age-related trends for protein levels in young children. Further, these analyses demonstrated that the serum levels of two peptides unique for apolipoprotein C1 (APOC1) were decreased after the appearance of the first islet autoantibody and remained relatively less abundant in children who progressed to type 1 diabetes, in comparison to autoantibody negative children.