Browsing by Author "Auvinen, Henni"

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  • Cellular delivery of enzyme-loaded DNA origami
    (2016) Ora, Ari; Järvihaavisto, Erika; Zhang, Hongbo; Auvinen, Henni; Santos, Hélder A.; Kostiainen, Mauri A.; Linko, Veikko
     School of Chemical Technology | A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
  • Electrostatic complexation of DNA nanostructures and biomacromolecules
    (2017-01-16) Auvinen, Henni
     Kemian tekniikan korkeakoulu | Master's thesis
    DNA origami folding technique allows the construction of nanoscale structures with almost any arbitrary shape or pattern. The self-assembly and the addressable surface makes the DNA origami method interesting in engineering novel functional materials and molecular-scale devices. However, the susceptibility to nuclease digestion in physiological conditions and poor transfection rates compromises their use in cellular applications, such as drug delivery vehicles. The aim of this thesis is to coat DNA origamis to improve their structural integrity and transfection rate. Two separate coating systems were studied, both based on the electrostatic and multivalent interactions between a negatively charged origami and positively charged biomacromolecules. The complexation of DNA origamis with the biomacromolecules was studied with agarose gel electrophoretic mobility shift assay and verified with transmission electron microscopy. The structural integrity of the coated origamis was studied by subjecting the origamis to enzymatic digestion by DNase I endonuclease. Finally, the transfection efficiency was studied using confocal microscopy and quantified with fluorescence-activated cell sorting. The results revealed successful coating of DNA origamis with the protein-dendron conjugate coating system. Two different proteins were used: bovine serum albumin (BSA) and hydrophobic I (HFBI). The proteins were attached to dendrons that served as the synthetic binding unit that attaches to the origami. BSA-coating conferred protection against nuclease digestion and enhanced the transfection efficiency into HEK293 cells. This work proposes a novel coating strategy that could find applications in sophisticated drug delivery and in other nucleotide-based bionanotechnological devices.
  • Fotoinitiaattorit lääketieteellisissä sovelluksissa
    (2013-05-07) Auvinen, Henni
     Sähkötekniikan korkeakoulu | Bachelor's thesis
  • Protein Coating of DNA Nanostructures for Enhanced Stability and Immunocompatibility
    (2017-09) Auvinen, Henni; Zhang, Hongbo; Nonappa, Nonappa; Kopilow, Alisa; Niemelä, Elina H.; Nummelin, Sami; Correia, Alexandra Mr; Santos, Hélder A.; Linko, Veikko; Kostiainen, Mauri A.
     A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
    Fully addressable DNA nanostructures, especially DNA origami, possess huge potential to serve as inherently biocompatible and versatile molecular platforms. However, their use as delivery vehicles in therapeutics is compromised by their low stability and poor transfection rates. This study shows that DNA origami can be coated by precisely defined one-to-one protein-dendron conjugates to tackle the aforementioned issues. The dendron part of the conjugate serves as a cationic binding domain that attaches to the negatively charged DNA origami surface via electrostatic interactions. The protein is attached to dendron through cysteine-maleimide bond, making the modular approach highly versatile. This work demonstrates the coating using two different proteins: bovine serum albumin (BSA) and class II hydrophobin (HFBI). The results reveal that BSA-coating significantly improves the origami stability against endonucleases (DNase I) and enhances the transfection into human embryonic kidney (HEK293) cells. Importantly,it is observed that BSA-coating attenuates the activation of immune response in mouse primary splenocytes. Serum albumin is the most abundant protein in the blood with a long circulation half-life and has already found clinically approved applications in drug delivery. It is therefore envisioned that the proposed system can open up further opportunities to tune the properties of DNA nanostructures in biological environment, and enable their use in various delivery applications.
  • Protein Coating of DNA Nanostructures for Enhanced Stability and Immunocompatibility
    (2017) Auvinen, Henni; Zhang, Hongbo; Nonappa, Nonappa; Kopilow, Alisa; Niemelä, Elina H.; Nummelin, Sami; Correia, Alexandra; Santos, Hélder A.; Linko, Veikko; Kostiainen, Mauri A.
     School of Science | A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä